| Headgroup-specific exposure of phospholipids in ABCA1-expressing cells. | |
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MedLine Citation:
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PMID: 15905177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ABCA1 has been established to be required for the efflux of cholesterol and phospholipids to apolipoproteins such as apoA-I. At present, it is unclear whether ABCA1-mediated lipid exposure is specific with regard to lipid headgroups and whether it requires calcium activation and the presence of a lipid acceptor. In the present work, we found exofacial exposure of endogenous phosphatidylserine in the absence of apoA-I to be enhanced in ABCA1-GFP expressing MDCKII and HeLa cells compared with control cells. By using C6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD)-labeled phospholipid analogues, we observed elevated redistribution of phosphatidylserine and phosphatidylethanolamine but not of phosphatidylcholine analogues from the cytoplasmic to the exoplasmic leaflet of the plasma membrane of ABCA1-GFP expressing cells. Whereas glyburide affected neither the level of exofacial endogenous PS nor the outward movement of the amino phospholipid analogues, the latter was sensitive to intracellular Ca2+ in ABCA1-GFP expressing cells, further enhancing outward analogue redistribution with respect to control cells. Both receptor-mediated endocytosis and fluidphase endocytosis were reduced in MDCKII cells expressing ABCA1-GFP. Glyburide raised the level of receptor-mediated endocytosis in the ABCA1-GFP expressing cell to the level of control cells in the absence of glyburide. In control cells, however, fluid-phase endocytosis but not receptor-mediated endocytosis was significantly reduced upon glyburide treatment. |
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Authors:
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Nele Alder-Baerens; Peter Müller; Antje Pohl; Thomas Korte; Yannick Hamon; Giovanna Chimini; Thomas Pomorski; Andreas Herrmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-05-19 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 280 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-11 Completed Date: 2005-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 26321-9 Citation Subset: IM |
Affiliation:
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Humboldt University of Berlin, Institute of Biology, Center of Biophysics and Bioinformatics, Invalidenstrasse 42, 10115 Berlin, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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chemistry*,
physiology* Animals Annexins / metabolism Apolipoproteins / chemistry Calcium / metabolism Cell Line Cytoplasm / metabolism Dogs Endocytosis Flow Cytometry Glyburide / chemistry Green Fluorescent Proteins / chemistry, metabolism Hela Cells Humans Kinetics Lipid Metabolism Lipids / chemistry Phospholipid Transfer Proteins / chemistry Phospholipids / chemistry* Protein Binding Protein Structure, Tertiary Temperature Time Factors Transferrin / chemistry |
| Chemical | |
Reg. No./Substance:
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0/ATP binding cassette transporter 1; 0/ATP-Binding Cassette Transporters; 0/Annexins; 0/Apolipoproteins; 0/Lipids; 0/Phospholipid Transfer Proteins; 0/Phospholipids; 10238-21-8/Glyburide; 11096-37-0/Transferrin; 147336-22-9/Green Fluorescent Proteins; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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