Document Detail


Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display.
MedLine Citation:
PMID:  16261155     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Head and neck squamous cell carcinoma (HNSCC) is common worldwide and is associated with a poor rate of survival. Identification of new markers and therapeutic targets, and understanding the complex transformation process, will require a comprehensive description of genome expression, that can only be achieved by combining different methodologies. We report here the HNSCC transcriptome that was determined by exhaustive differential display (DD) analysis coupled with validation by different methods on the same patient samples. The resulting 820 nonredundant sequences were analysed by high throughput bioinformatics analysis. Human proteins were identified for 73% (596) of the DD sequences. A large proportion (>50%) of the remaining unassigned sequences match ESTs (expressed sequence tags) from human tumours. For the functionally annotated proteins, there is significant enrichment for relevant biological processes, including cell motility, protein biosynthesis, stress and immune responses, cell death, cell cycle, cell proliferation and/or maintenance and transport. Three of the novel proteins (TMEM16A, PHLDB2 and ARHGAP21) were analysed further to show that they have the potential to be developed as therapeutic targets.
Authors:
A Carles; R Millon; A Cromer; G Ganguli; F Lemaire; J Young; C Wasylyk; D Muller; I Schultz; Y Rabouel; D Dembélé; C Zhao; P Marchal; C Ducray; L Bracco; J Abecassis; O Poch; B Wasylyk
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  25     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2006-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  1821-31     Citation Subset:  IM    
Affiliation:
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, 67404 Illkirch Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Blotting, Northern
Carcinoma, Squamous Cell / genetics*
Computational Biology
DNA, Neoplasm / analysis*
Gene Expression
Gene Expression Profiling / methods*
Genomics
Head and Neck Neoplasms / genetics*
Humans
Immunohistochemistry
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis*
Protein Array Analysis*
Proteome
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA / methods
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Proteome

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