Document Detail

Head and neck squamous cell carcinoma is highly sensitive to vinorelbine in Vitro.
MedLine Citation:
PMID:  12530056     Owner:  NLM     Status:  MEDLINE    
The vinorelbine sensitivity of eight recently established head and neck squamous cell carcinoma (SCC) cell lines was tested using the 96-well plate clonogenic assay. The chemosensitivity of these head and neck SCC cell lines to vinorelbine expressed as IC50, corresponding to the drug concentration causing 50% inhibition in clonogenic survival, varied between 0.6 and 1.0 nM. The dose-dependent growth inhibition caused by vinorelbine was measured in three of these cell lines. A clear growth inhibition was observed at a concentration of 3 nM. The same cell lines were studied with flow cytometry. When exposed to 3 nM and 5 nM vinorelbine, an accumulation of the cells in the G2/M-phase was observed in all cultures after 12 hours. The morphological changes induced by 3 nM and 5 nM vinorelbine to the UT-SCC-33 cell line were analysed with time-lapse video microscopy. In the cultures treated with 5 nM vinorelbine, the cells stayed mitotically arrested for 2-32 hours and thereafter died morphologically by apoptosis. These results indicate that, in vitro, the head and neck SCC is consistently sensitive to vinorelbine, which blocks the cell cycle in G2/M, the most radiosensitive phase. These encouraging results suggest that vinorelbine may potentially be used in conjunction with radiotherapy in the treatment of head and neck SCC.
Kaisa Erjala; Jaakko Pulkkinen; Jarmo Kulmala; Kalle Alanen; Reidar Grenman
Related Documents :
10899676 - Type i collagen degradation by invasive oral squamous cell carcinoma.
1418896 - A study of the heterogeneity of the mucoepidermoid tumor and the implication for future...
1692016 - A novel monoclonal antibody against squamous cell carcinoma.
12165056 - Subcellular localization of beta-catenin in malignant cell lines and squamous cell carc...
23007466 - Gene expression profiling for mechanistic understanding of cellular aggregation in mamm...
22919666 - Bacterial toxin effector-membrane targeting: outside in, then back again.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  22     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2002 Nov-Dec
Date Detail:
Created Date:  2003-01-17     Completed Date:  2003-01-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3135-42     Citation Subset:  IM    
Department of Otorhinolaryngology-Head and Neck Surgery, Turku University, Central Hospital, FIN-20520 Turku, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Carcinoma, Squamous Cell / drug therapy*,  pathology
Cell Division / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Flow Cytometry
G2 Phase / drug effects
Head and Neck Neoplasms / drug therapy*,  pathology
Inhibitory Concentration 50
Mitosis / drug effects
Tumor Cells, Cultured
Vinblastine / analogs & derivatives*,  pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 71486-22-1/vinorelbine; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of the isoprenoids perillyl alcohol and farnesol on apoptosis biomarkers in pancreatic cance...
Next Document:  Combined effect of hyperthermia at 42 degrees C and irradiation dose of 2 Gy on two rat yolk sac tum...