Document Detail


Head and neck squamous cell carcinoma is highly sensitive to vinorelbine in Vitro.
MedLine Citation:
PMID:  12530056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The vinorelbine sensitivity of eight recently established head and neck squamous cell carcinoma (SCC) cell lines was tested using the 96-well plate clonogenic assay. The chemosensitivity of these head and neck SCC cell lines to vinorelbine expressed as IC50, corresponding to the drug concentration causing 50% inhibition in clonogenic survival, varied between 0.6 and 1.0 nM. The dose-dependent growth inhibition caused by vinorelbine was measured in three of these cell lines. A clear growth inhibition was observed at a concentration of 3 nM. The same cell lines were studied with flow cytometry. When exposed to 3 nM and 5 nM vinorelbine, an accumulation of the cells in the G2/M-phase was observed in all cultures after 12 hours. The morphological changes induced by 3 nM and 5 nM vinorelbine to the UT-SCC-33 cell line were analysed with time-lapse video microscopy. In the cultures treated with 5 nM vinorelbine, the cells stayed mitotically arrested for 2-32 hours and thereafter died morphologically by apoptosis. These results indicate that, in vitro, the head and neck SCC is consistently sensitive to vinorelbine, which blocks the cell cycle in G2/M, the most radiosensitive phase. These encouraging results suggest that vinorelbine may potentially be used in conjunction with radiotherapy in the treatment of head and neck SCC.
Authors:
Kaisa Erjala; Jaakko Pulkkinen; Jarmo Kulmala; Kalle Alanen; Reidar Grenman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  22     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2002 Nov-Dec
Date Detail:
Created Date:  2003-01-17     Completed Date:  2003-01-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3135-42     Citation Subset:  IM    
Affiliation:
Department of Otorhinolaryngology-Head and Neck Surgery, Turku University, Central Hospital, FIN-20520 Turku, Finland.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*
Carcinoma, Squamous Cell / drug therapy*,  pathology
Cell Division / drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Female
Flow Cytometry
G2 Phase / drug effects
Head and Neck Neoplasms / drug therapy*,  pathology
Humans
Inhibitory Concentration 50
Male
Mitosis / drug effects
Tumor Cells, Cultured
Vinblastine / analogs & derivatives*,  pharmacology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 71486-22-1/vinorelbine; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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