| Head and neck cancer cell lines are resistant to mitochondrial-depolarization-induced apoptosis. | |
| | |
MedLine Citation:
|
PMID: 18483464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Mitochondrial dysfunction has been linked to defects in the apoptotic pathway, and solid tumors, including head and neck squamous cell carcinoma (HNSCC), exhibit defects in apoptosis. Loss of mitochondrial membrane potential (DeltaPsim) is an early initiating event in the mitochondrial apoptotic pathway. We investigated the apoptotic response of 3 head and neck cancer cell lines treated with a mitochondrial-membrane-depolarizing agent, valinomycin, and studied the ability of depolarization to induce release of cytochrome c in these cell lines. EXPERIMENTAL DESIGN: HNSCC cell lines JHU-011, -012 and -019, and a leukemia control cell line HL-60 were assayed for DeltaPsim after valinomycin treatment by staining with mitochondrial-membrane-potential-specific probe JC-1 and stained with apoptosis-specific probe annexin-V to measure their rate of apoptosis by FACS. Western blotting was also applied to detect cytoplasmic cytochrome c release. RESULTS: DeltaPsim in head and neck cell lines started to show slight loss of DeltaPsim, while HL-60 showed significant loss of DeltaPsim after 30 min of treatment. All cell lines demonstrated complete mitochondrial depolarization within 24 h, however, only the control cell line HL-60 underwent apoptosis. In addition, HNSCC cell lines did not demonstrate cytoplasmic cytochrome c release despite significant mitochondrial membrane depolarization, while HL-60 cell initiated apoptosis and cytochcrome c release after 24 h of treatment. CONCLUSIONS: Head and neck cancer cell lines exhibit defects in mitochondrial-membrane-depolarization-induced apoptosis as well as impaired release of cytochrome c despite significant mitochondrial membrane depolarization. Proximal defects in the mitochondrial apoptosis pathway are a feature of HNSCC. |
| | |
Authors:
|
Ming Zhao; Wojciech K Mydlarz; Shaoyu Zhou; Joseph Califano |
Related Documents
:
|
20856874 - Prohibitins are required for cancer cell proliferation and adhesion. 1383244 - Mitochondrial gene expression in the human gastrointestinal tract. 2157694 - Stereological and functional investigations on isolated adrenocortical cells. iii. zona... 15246834 - Nitric oxide production and regulation of neuronal nos in tyrosine hydroxylase containi... 10705394 - Salivary gland basal cell and canalicular adenomas: immunohistochemical demonstration o... 22240484 - Autophagy releases lipid that promotes fibrogenesis by activated hepatic stellate cells... |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2008-05-16 |
Journal Detail:
|
Title: ORL; journal for oto-rhino-laryngology and its related specialties Volume: 70 ISSN: 1423-0275 ISO Abbreviation: ORL J. Otorhinolaryngol. Relat. Spec. Publication Date: 2008 |
Date Detail:
|
Created Date: 2008-06-20 Completed Date: 2008-08-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0334721 Medline TA: ORL J Otorhinolaryngol Relat Spec Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 257-63 Citation Subset: IM |
Copyright Information:
|
Copyright 2008 S. Karger AG, Basel. |
Affiliation:
|
Department of Otolaryngology, Head and Neck Surgery, Johns Hopkins Medical Institution, Baltimore, MD 21287-0910, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
physiology* Blotting, Western Carcinoma, Squamous Cell / metabolism, pathology* Cell Line, Tumor Cytochromes c / biosynthesis Flow Cytometry Head and Neck Neoplasms / metabolism, pathology* Humans Membrane Potential, Mitochondrial / physiology* Mitochondria / metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
|
9007-43-6/Cytochromes c |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of life-threatening complications of deep neck abscess and the impact of empiric antibiotic...
Next Document: TNF alpha-induced down-regulation of estrogen receptor alpha in MCF-7 breast cancer cells.