Document Detail


HeLa Cell Transfection Using a Novel Sonoporation System.
MedLine Citation:
PMID:  20977982     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, <formula formulatype="inline"><tex Notation="TeX">$p$</tex></formula> &lt; 0.05) at the resonant frequency of 980 kHz at 100 V<formula formulatype="inline"><tex Notation="TeX">$_{\rm{p-p}}$</tex></formula> (19.5 MPa) with a cell viability of 77% after 10 s of insonication.
Authors:
Somphop Rodamporn; N R Harris; Steve P Beeby; Rosemary J Boltryk; Tilman Sanchez-Elsner
Publication Detail:
Type:  Journal Article     Date:  2010-10-25
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  58     ISSN:  1558-2531     ISO Abbreviation:  IEEE Trans Biomed Eng     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  927-34     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Human-robot synchrony: flexible assistance using adaptive oscillators.
Next Document:  Algorithm-enabled low-dose micro-CT imaging.