| HeLa Cell Transfection Using a Novel Sonoporation System. | |
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MedLine Citation:
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PMID: 20977982 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, <formula formulatype="inline"><tex Notation="TeX">$p$</tex></formula> < 0.05) at the resonant frequency of 980 kHz at 100 V<formula formulatype="inline"><tex Notation="TeX">$_{\rm{p-p}}$</tex></formula> (19.5 MPa) with a cell viability of 77% after 10 s of insonication. |
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Authors:
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Somphop Rodamporn; N R Harris; Steve P Beeby; Rosemary J Boltryk; Tilman Sanchez-Elsner |
Publication Detail:
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Type: Journal Article Date: 2010-10-25 |
Journal Detail:
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Title: IEEE transactions on bio-medical engineering Volume: 58 ISSN: 1558-2531 ISO Abbreviation: IEEE Trans Biomed Eng Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0012737 Medline TA: IEEE Trans Biomed Eng Country: United States |
Other Details:
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Languages: eng Pagination: 927-34 Citation Subset: IM |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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