Document Detail


Has the bacterial biphenyl catabolic pathway evolved primarily to degrade biphenyl? The diphenylmethane case.
MedLine Citation:
PMID:  23749969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, we have compared the ability of Pandoraea pnomenusa B356 and of Burkholderia xenovorans LB400 to metabolize diphenylmethane and benzophenone, two biphenyl analogs in which the phenyl rings are bonded to a single carbon. Both chemicals are of environmental concern. P. pnomenusa B356 grew well on diphenylmethane. On the basis of growth kinetics analyses, diphenylmethane and biphenyl were shown to induce the same catabolic pathway. The profile of metabolites produced during growth of strain B356 on diphenylmethane was the same as the one produced by isolated enzymes of the biphenyl catabolic pathway acting individually or in coupled reactions. The biphenyl dioxygenase oxidizes diphenylmethane to 3-benzylcyclohexa-3,5-diene-1,2-diol very efficiently, and ultimately this metabolite is transformed to phenylacetic acid, which is further metabolized by a lower pathway. Strain B356 was also able to cometabolize benzophenone through its biphenyl pathway, although in this case, this substrate was unable to induce the biphenyl catabolic pathway and the degradation was incomplete, with accumulation of 2-hydroxy-6,7-dioxo-7-phenylheptanoic acid. Unlike strain B356, B. xenovorans LB400 did not grow on diphenylmethane. Its biphenyl pathway enzymes metabolized diphenylmethane, but they poorly metabolize benzophenone. The fact that the biphenyl catabolic pathway of strain B356 metabolized diphenylmethane and benzophenone more efficiently than that of strain LB400 brings us to postulate that in strain B356, this pathway evolved divergently to serve other functions not related to biphenyl degradation.
Authors:
Thi Thanh My Pham; Michel Sylvestre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-06-07
Journal Detail:
Title:  Journal of bacteriology     Volume:  195     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-26     Completed Date:  2013-10-18     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3563-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Benzhydryl Compounds / metabolism*,  pharmacology
Benzophenones / metabolism*,  pharmacology
Biphenyl Compounds / chemistry,  metabolism*
Burkholderiaceae / genetics,  metabolism*
Gene Expression Regulation, Bacterial / physiology
Models, Molecular
Molecular Structure
Chemical
Reg. No./Substance:
0/Benzhydryl Compounds; 0/Benzophenones; 0/Biphenyl Compounds; 2L9GJK6MGN/diphenyl; 701M4TTV9O/benzophenone; K3E387I0BC/diphenylmethane
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