| Harmonic analysis of the cone flicker ERG of rabbit. | |
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MedLine Citation:
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PMID: 20974130 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Harmonic analysis was used to characterize the rabbit flicker ERG elicited by sinusoidally modulated full-field stimuli under light-adapted conditions. The frequency-response function for fundamental amplitude, derived from Fourier analysis of the ERG waveforms, exhibited two limbs, with an amplitude minimum at approximately 30Hz, and a high-frequency region peaking at around 45Hz and extending to more than 100Hz at higher adapting levels. At low frequencies (<20Hz), the fundamental response amplitude was independent of mean luminance (Weber law behavior), whereas the response amplitude at high stimulus frequencies varied nonlinearly with mean luminance. At low frequencies, intravitreal administration of L-AP4, which blocks ON-pathway activity, reduced the fundamental response amplitude and produced a phase shift. On the other hand, PDA, which reduces OFF-pathway activity, had a minimal effect on both the response amplitude and phase at low frequencies. At high frequencies, L-AP4 increased the fundamental response amplitude at low mean luminances, whereas PDA had only a small effect on amplitude and phase. Both pharmacologic agents removed the minimum in the amplitude-frequency function as well as the abrupt change in phase at stimulus frequencies near 30Hz. The results suggest that there is a nonlinear interaction between ON- and OFF-pathway activity over the entire stimulus frequency range examined in this study. These findings provide a basis for formulating protocols to evaluate the effect of pharmacologic agents and/or disease on the cone flicker ERG of rabbit. |
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Authors:
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Haohua Qian; Kenneth R Alexander; Harris Ripps |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-23 |
Journal Detail:
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Title: Experimental eye research Volume: 91 ISSN: 1096-0007 ISO Abbreviation: Exp. Eye Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2010-12-30 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 811-7 Citation Subset: IM |
Copyright Information:
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Published by Elsevier Ltd. |
Affiliation:
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Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA. haohua.qian@nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Ocular Aminobutyric Acids / pharmacology Animals Electroretinography* Excitatory Amino Acid Agonists / pharmacology Excitatory Amino Acid Antagonists / pharmacology Fourier Analysis Intravitreal Injections Male Photic Stimulation Pipecolic Acids / pharmacology Rabbits Retinal Cone Photoreceptor Cells / drug effects, physiology*, radiation effects Retinal Neurons / physiology |
| Grant Support | |
ID/Acronym/Agency:
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EY008301/EY/NEI NIH HHS; EY01792/EY/NEI NIH HHS; P30 EY001792-33/EY/NEI NIH HHS; R01 EY008301-20/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aminobutyric Acids; 0/Excitatory Amino Acid Agonists; 0/Excitatory Amino Acid Antagonists; 0/Pipecolic Acids; 46026-75-9/2,3-piperidinedicarboxylic acid; 6323-99-5/2-amino-4-phosphonobutyric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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