| Hapten-specific T lymphocyte activation by glutaraldehyde-treated macrophages: an argument against antigen processing by macrophages. | |
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MedLine Citation:
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PMID: 152327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this communication the effects of glutaraldehyde treatment of trinitrophenyl-(TNP) modified macrophages on their ability to stimulate TNP-specific guinea pig T lymphocyte proliferation were studied. TNP-modified macrophages briefly treated with glutaraldehyde retained much of their ability to stimulate TNP-primed T cells. In contrast, similar treatment of allogeneic macrophages or soluble protein antigen-pulsed syngeneic macrophages completely eliminated their ability to stimulate a mixed leukocyte reaction or protein antigen-specific proliferation, respectively. TNP-modification did not appear to interfere with glutaraldehyde reactivity since macrophages treated with glutaraldehyde before or after TNP-modification stimulated equivalent T cell responses. However, glutaraldehyde treatment of TNP-modified macrophages that had been cultured overnight dramatically reduced their ability to stimulate TNP-specific T cells. Glutaraldehyde-treated TNP-modified macrophages also expressed the same genetic restrictions of T cell activation as untreated stimulators. Thus, T cells primed with syngeneic TNP-modified macrophages were restimulated only by glutaraldehyde-treated TNP-modified syngeneic, but not by allogeneic, macrophages. These results are discussed with respect to the nature of the TNP-specific immunogen recognized by T cells. |
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Authors:
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D W Thomas |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 121 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1978 Nov |
Date Detail:
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Created Date: 1979-01-15 Completed Date: 1979-01-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1760-6 Citation Subset: AIM; IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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pharmacology* Animals Antigens* / genetics Cell Survival Glutaral / pharmacology* Guinea Pigs Haptens* Immune Sera / pharmacology Lymphocyte Culture Test, Mixed Macrophages / immunology* T-Lymphocytes / immunology* Trinitrobenzenes / immunology |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Antigens; 0/Haptens; 0/Immune Sera; 0/Trinitrobenzenes; 111-30-8/Glutaral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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