Document Detail

Hapten-specific T-cell response to azobenzenearsonate-N-acetyl-L-tyrosine in the Lewis rat. IV. Rat dendritic cells present soluble and insoluble azobenzenearsonate conjugates to T cells.
MedLine Citation:
PMID:  2408768     Owner:  NLM     Status:  MEDLINE    
We have used a fractionation procedure involving bovine serum albumin gradient floatation, adherence to glass, and rosetting with antibody-coated sheep erythrocytes to purify an accessory cell fraction from Lewis rat spleens. The fraction which is of light buoyant density, nonadherent, and FcR- is markedly Ia+, lacks phagocytic ability, is nonspecific esterase negative and under scanning electron microscopy has a heterogeneous morphology with a variety of protuberences described for rat dendritic cells. This putative dendritic cell preparation is very effective at stimulating proliferative responses with concanavalin A and allogeneic cells. When used to reconstitute the reactivity of peritoneal exudate cells of rats immunized with azobenzenearsonate-N-acetyl-L-tyrosine (ABA-Tyr) and subsequently depleted of Ia+ cells, it was shown to be highly effective with ABA conjugates of tyrosine, Ficoll, and polystyrene beads. Thus, despite the apparent absence of phagocytic or degradative abilities, this cell was very efficient at presenting large soluble and insoluble antigens. It is suggested that processing may occur at the cell surface without requiring internalization.
A Amsden; C Y Lawn; S Roy; G H Sunshine; S Leskowitz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  93     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-08-19     Completed Date:  1985-08-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  447-58     Citation Subset:  IM    
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MeSH Terms
Antigen-Presenting Cells / immunology
Azo Compounds / immunology*
Cell Separation
Haptens / immunology*
Lymphoid Tissue / cytology
Rats, Inbred Lew
T-Lymphocytes / immunology*
Tyrosine / analogs & derivatives*,  immunology
p-Azobenzenearsonate / analogs & derivatives,  immunology*
Grant Support
Reg. No./Substance:
0/Azo Compounds; 0/Epitopes; 0/Haptens; 39927-13-4/azobenzenearsonate-N-acetyl-L-tyrosine; 55520-40-6/Tyrosine; 7334-23-8/p-Azobenzenearsonate

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