|Hantavirus in bat, Sierra Leone.|
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|PMID: 22261176 Owner: NLM Status: MEDLINE|
|Sabrina Weiss; Peter T Witkowski; Brita Auste; Kathrin Nowak; Natalie Weber; Jakob Fahr; Jean-Vivien Mombouli; Nathan D Wolfe; Jan Felix Drexler; Christian Drosten; Boris Klempa; Fabian H Leendertz; Detlev H Kruger|
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|Type: Letter; Research Support, Non-U.S. Gov't|
|Title: Emerging infectious diseases Volume: 18 ISSN: 1080-6059 ISO Abbreviation: Emerging Infect. Dis. Publication Date: 2012 Jan|
|Created Date: 2012-01-20 Completed Date: 2012-05-15 Revised Date: 2013-05-22|
Medline Journal Info:
|Nlm Unique ID: 9508155 Medline TA: Emerg Infect Dis Country: United States|
|Languages: eng Pagination: 159-61 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
DNA, Complementary / genetics
Hantaan virus / genetics*, isolation & purification*
Hantavirus Infections / epidemiology, veterinary*, virology
RNA, Viral / genetics
Sierra Leone / epidemiology
|0/DNA, Complementary; 0/RNA, Viral|
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (publisher-id): EID
Publisher: Centers for Disease Control and Prevention
Print publication date: Month: 1 Year: 2012
Volume: 18 Issue: 1
First Page: 159 Last Page: 161
PubMed Id: 22261176
Publisher Id: 11-1026
|Hantavirus in Bat, Sierra Leone Alternate Title:Hantavirus in Bat, Sierra Leone|
|Peter T. Witkowski1|
|Nathan D. Wolfe|
|Jan Felix Drexler|
|Fabian H. Leendertz|
|Detlev H. Kruger|
|Robert Koch-Institute, Berlin, Germany (S. Weiss, K. Nowak, F.H. Leendertz);
|Charité School of Medicine, Berlin (P.T. Witkowski, B. Auste, B. Klempa, D.H. Kruger);
|Ulm University, Ulm, Germany (N. Weber);
|University of Braunschweig, Braunschweig, Germany (J. Fahr);
|Laboratoire National de Santé Publique, Brazzaville, Republic of the Congo (J.-V. Mombouli);
|Stanford University Program in Human Biology, Stanford, California, USA (N.D. Wolfe);
|University of Bonn Medical Centre, Bonn, Germany (J.F. Drexler, C. Drosten);
|Slovak Academy of Sciences, Bratislava, Slovakia (B. Klempa)
|Correspondence: Address for correspondence: Detlev H. Kruger, Institute of Medical Virology, Helmut-Ruska-Haus, Charité Medical School, Charitéplatz 1, D-10117 Berlin, Germany; email: firstname.lastname@example.org
To the Editor: Hantaviruses (family Bunyaviridae) are transmitted from rodent reservoirs to humans. These viruses cause life-threatening human diseases: hantavirus cardiopulmonary syndrome in the Americas and hemorrhagic fever with renal syndrome in Asia and Europe (1). Since 2006, indigenous hantaviruses were reported also from Africa. Sangassou virus was found in an African wood mouse (Hylomyscus simus) in Guinea (2). Discovery of newer African hantaviruses, Tanganya virus and recently Azagny virus, was even more surprising because they were found in shrews (3,4).
The detection of hantaviruses in small mammals other than rodents, such as shrews and also moles (4), increasingly raises questions regarding the real hantavirus host range. Bats (order Chiroptera) are already known to harbor a broad variety of emerging pathogens, including other bunyaviruses (5). Their ability to fly and social life history enable efficient pathogen maintenance, evolution, and spread. Therefore, we conducted a study on hantaviruses in bats from Africa.
A total of 525 tissue samples from 417 bats representing 28 genera were tested for the presence of hantavirus RNA. Samples originated from different regions in western and central Africa and were collected during 2009 and early 2011. Total RNA was extracted from tissue samples and reverse transcribed. cDNA was screened by PCR specific for sequences of the large genomic segment across the genus Hantavirus (2).
One sample yielded a product of the expected size and was subjected to cloning and sequencing. The positive sample (MGB/1209) was obtained from 1 of 18 investigated slit-faced bats (family Nycteridae). The animal was trapped at the Magboi River within Gola National Park, Sierra Leone (7°50.194′N, 10°38.626′W), and the identification as Nycteris hispida has been verified with the voucher specimen (RCJF529). Histologic examination of organs of the animal showed no obvious pathologic findings.
The obtained 414-nt sequence covers a genomic region, which was found to correspond to nt position 2,918–3,332 in the large segment open reading frame of prototypic Hantaan virus. Bioinformatic analysis on the amino acid level showed highest degrees of identity to shrew- and mole-associated hantaviruses (Thottapalayam virus 73.0%, Altai virus 69.7%, Nova and Imjin virus 69.3%). On the basis of tree topology of a maximum-likelihood phylogenetic tree, the sequence does not cluster with rodent-associated hantaviruses but groups with those found in shrews and moles (Figure).
Considering that bats are more closely related to shrews and moles than to rodents (6), a certain genetic similarity of a putative bat-borne hantavirus with shrew- and mole-associated hantaviruses seems reasonable. Notably, shrew-associated Thottapalayam virus (India) and Imjin virus (South Korea) seem to be closer relatives, and African Tanganya virus (Guinea) and Azagny virus (Côte d’Ivoire) are more distantly related. Additional sequence data is needed for more conclusive phylogenetic analyses.
Because the new amino acid sequence is at least 22% divergent from those of other hantaviruses, we conclude that the bat was infected with a newly found hantavirus. We propose the putative name Magboi virus (MGBV) for the new virus because it was detected in an animal captured at the Magboi River in Sierra Leone. The MGBV nucleotide sequence is novel and has not been known or handled before in our laboratory. Before this study, hantavirus nucleic acid was found in lung and kidney tissues of bats from the genera Eptesicus and Rhinolophus in South Korea. However, nucleotide sequencing showed the presence of prototypical Hantaan virus indicating a spillover infection or laboratory contamination (7).
Further screening is necessary to confirm N.hispida as a natural reservoir host of the virus. Although the presented bat-associated sequence is obviously distinct from other hantaviruses, which suggests association with a novel natural host, a spillover infection from another, yet unrecognized host cannot be ruled out. However, detection of the virus exclusively in 1 organ (lung but not in liver, kidney, and spleen; data not shown) suggests a persistent infection that is typically observed in natural hosts of hantaviruses (8).
To date, only a few reports exist on cases of hemorrhagic fever with renal syndrome in Africa (9,10). However, underreporting must be assumed because the symptoms resemble those of many other febrile infections. Moreover, in cases of infections by non–rodent-associated hantaviruses, cross-reactivity with routinely used rodent-borne virus antigens should be limited and may hamper human serodiagnostics (1). The results suggest that bats, which are hosts of many emerging pathogens (5), may act as natural reservoirs for hantavirus. The effect of this virus on public health remains to be determined.
Suggested citation for this article: Weiss S, Witkowski PT, Auste B, Nowak K, Weber N, Fahr J, et al. Hantavirus in bat, Sierra Leone [letter]. Emerg Infect Dis [serial on the Internet]. 2012 Jan [date cited]. http://dx.doi.org/10.3201/eid1801.111026
1These authors contributed equally to this article.
This study was supported by Deutsche Forschungsgemeinschaft (grant KR1293/9-1). Work in Sierra Leone was supported by the Gola Forest Project. National and local authorities in Sierra Leone, Liberia, Mali, Senegal, and Republic of Congo kindly granted research and export permits. N.D.W. is supported by an award from the National Institutes of Health Director’s Pioneer Award (grant DP1-OD000370).
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|7. .||JungYT, KimGRGenomic characterization of M and S RNA segments of hantaviruses isolated from bats.Acta Virol. Year: 1995;39:231–38825306|
|8. .||SchönrichG, RangA, LuttekeN, RafteryMJ, CharbonnelN, UlrichRGHantavirus-induced immunity in rodent reservoirs and humans.Immunol Rev. Year: 2008;225:163–8910.1111/j.1600-065X.2008.00694.x18837782|
|9. .||CoulaudX, ChouaibE, GeorgesAJ, RollinP, GonzalezJPFirst human case of haemorrhagic fever with renal syndrome in the Central African Republic.Trans R Soc Trop Med Hyg. Year: 1987;81:68610.1016/0035-9203(87)90455-X2895515|
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Keywords: Keywords: hantavirus, rodent-borne pathogens, bats, Africa, Chiroptera, Sierra Leone, zoonosis.
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