Document Detail

A HaloTag-based small molecule microarray screening methodology with increased sensitivity and multiplex capabilities.
MedLine Citation:
PMID:  23013033     Owner:  NLM     Status:  MEDLINE    
Small Molecule Microarrays (SMMs) represent a general platform for screening small molecule-protein interactions independent of functional inhibition of target proteins. In an effort to increase the scope and utility of SMMs, we have modified the SMM screening methodology to increase assay sensitivity and facilitate multiplex screening. Fusing target proteins to the HaloTag protein allows us to covalently prelabel fusion proteins with fluorophores, leading to increased assay sensitivity and an ability to conduct multiplex screens. We use the interaction between FKBP12 and two ligands, rapamycin and ARIAD's "bump" ligand, to show that the HaloTag-based SMM screening methodology significantly increases assay sensitivity. Additionally, using wild type FKBP12 and the FKBP12 F36V mutant, we show that prelabeling various protein isoforms with different fluorophores allows us to conduct multiplex screens and identify ligands to a specific isoform. Finally, we show this multiplex screening technique is capable of identifying ligands selective for a specific PTP1B isoform using a 20,000 compound screening deck.
Devin J Noblin; Charlotte M Page; Hyun Seop Tae; Peter C Gareiss; John S Schneekloth; Craig M Crews
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Publication Detail:
Type:  Journal Article     Date:  2012-10-11
Journal Detail:
Title:  ACS chemical biology     Volume:  7     ISSN:  1554-8937     ISO Abbreviation:  ACS Chem. Biol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-21     Completed Date:  2013-06-04     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  101282906     Medline TA:  ACS Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2055-63     Citation Subset:  IM    
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MeSH Terms
Base Sequence
DNA Primers
Protein Array Analysis*
Small Molecule Libraries*
Tacrolimus Binding Protein 1A / chemistry*
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/Small Molecule Libraries; EC 5.2.1.-/Tacrolimus Binding Protein 1A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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