Document Detail


The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.
MedLine Citation:
PMID:  23217709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lysosome is a degradative organelle, and its fusion with other organelles is strictly regulated. In contrast to fusion with the late endosome, the mechanisms underlying autophagosome-lysosome fusion remain unknown. Here, we identify syntaxin 17 (Stx17) as the autophagosomal SNARE required for fusion with the endosome/lysosome. Stx17 localizes to the outer membrane of completed autophagosomes but not to the isolation membrane (unclosed intermediate structures); for this reason, the lysosome does not fuse with the isolation membrane. Stx17 interacts with SNAP-29 and the endosomal/lysosomal SNARE VAMP8. Depletion of Stx17 causes accumulation of autophagosomes without degradation. Stx17 has a unique C-terminal hairpin structure mediated by two tandem transmembrane domains containing glycine zipper-like motifs, which is essential for its association with the autophagosomal membrane. These findings reveal a mechanism by which the SNARE protein is available to the completed autophagosome.
Authors:
Eisuke Itakura; Chieko Kishi-Itakura; Noboru Mizushima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  151     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  2013-02-06     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1256-69     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Amino Acid Sequence
Animals
Autophagy
Cell Line
Cytosol / metabolism
Endosomes / metabolism*
Fibroblasts / metabolism
HEK293 Cells
HeLa Cells
Humans
Lysosomes / metabolism*
Mice
Molecular Sequence Data
Phagosomes / metabolism*
Protein Structure, Tertiary
Qa-SNARE Proteins / chemistry,  metabolism*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Qa-SNARE Proteins; 0/STX17 protein, human; 0/Stx17 protein, mouse
Comments/Corrections
Comment In:
Nat Rev Mol Cell Biol. 2013 Feb;14(2):65   [PMID:  23249901 ]

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