| The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. | |
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MedLine Citation:
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PMID: 23217709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lysosome is a degradative organelle, and its fusion with other organelles is strictly regulated. In contrast to fusion with the late endosome, the mechanisms underlying autophagosome-lysosome fusion remain unknown. Here, we identify syntaxin 17 (Stx17) as the autophagosomal SNARE required for fusion with the endosome/lysosome. Stx17 localizes to the outer membrane of completed autophagosomes but not to the isolation membrane (unclosed intermediate structures); for this reason, the lysosome does not fuse with the isolation membrane. Stx17 interacts with SNAP-29 and the endosomal/lysosomal SNARE VAMP8. Depletion of Stx17 causes accumulation of autophagosomes without degradation. Stx17 has a unique C-terminal hairpin structure mediated by two tandem transmembrane domains containing glycine zipper-like motifs, which is essential for its association with the autophagosomal membrane. These findings reveal a mechanism by which the SNARE protein is available to the completed autophagosome. |
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Authors:
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Eisuke Itakura; Chieko Kishi-Itakura; Noboru Mizushima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell Volume: 151 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-10 Completed Date: 2013-02-06 Revised Date: 2013-03-04 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1256-69 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Amino Acid Sequence Animals Autophagy Cell Line Cytosol / metabolism Endosomes / metabolism* Fibroblasts / metabolism HEK293 Cells HeLa Cells Humans Lysosomes / metabolism* Mice Molecular Sequence Data Phagosomes / metabolism* Protein Structure, Tertiary Qa-SNARE Proteins / chemistry, metabolism* Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Qa-SNARE Proteins; 0/STX17 protein, human; 0/Stx17 protein, mouse |
| Comments/Corrections | |
Comment In:
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Nat Rev Mol Cell Biol. 2013 Feb;14(2):65
[PMID:
23249901
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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