Document Detail

Hair follicle-derived smooth muscle cells and small intestinal submucosa for engineering mechanically robust and vasoreactive vascular media.
MedLine Citation:
PMID:  21083418     Owner:  NLM     Status:  MEDLINE    
Our laboratory recently reported a new source of smooth muscle cells (SMCs) derived from hair follicle (HF) mesenchymal stem cells. HF-SMCs demonstrated high proliferation and clonogenic potential as well as contractile function. In this study, we aimed at engineering the vascular media using HF-SMCs and a natural biomaterial, namely small intestinal submucosa (SIS). Engineering functional vascular constructs required application of mechanical force, resulting in actin reorganization and cellular alignment. In turn, cell alignment was necessary for development of receptor- and nonreceptor-mediated contractility as soon as 24 h after cell seeding. Within 2 weeks in culture, the cells migrated into SIS and secreted collagen and elastin, the two major extracellular matrix components of the vessel wall. At 2 weeks, vascular reactivity increased significantly up to three- to fivefold and mechanical properties were similar to those of native ovine arteries. Taken together, our data demonstrate that the combination of HF-SMCs with SIS resulted in mechanically strong, biologically functional vascular media with potential for arterial implantation.
Hao-Fan Peng; Jin Yu Liu; Stelios T Andreadis; Daniel D Swartz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-01-16
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  17     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-23     Completed Date:  2011-08-03     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  981-90     Citation Subset:  IM    
Department of Chemical and Biological Engineering, Women and Children's Hospital of Buffalo, University at Buffalo, State University of New York, Amherst, New York 14260-4200, USA.
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MeSH Terms
Cells, Cultured
Hair Follicle / cytology*,  ultrastructure
Intestine, Small / cytology*,  ultrastructure
Microscopy, Electron, Scanning
Myocytes, Smooth Muscle / cytology*,  ultrastructure
Tissue Engineering / methods*
Tunica Media / cytology*,  ultrastructure
Grant Support

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