| Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry. | |
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MedLine Citation:
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PMID: 15386502 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for the determination of nicotine and cotinine in human hair was established. In the procedure, a hair sample (10 mg) was washed with dichloromethane and digested in 2.5 M sodium hydroxide. The digest was extracted with dichloromethane and then 25 mM hydrochloric acid in methanol was added to the extract, to prevent loss of analytes. The solution was evaporated and redissolved in the mobile phase, methanol/10 mM ammonium acetate (30/70, v/v). A 20 microL aliquot of redissolved solution was subjected to analysis. Nicotine and cotinine in human hair were quantified by using deuterated analytes as internal standards. The quantification limits were 8 microg/L for nicotine and 0.9 microg/L for cotinine. The proposed method was applied to measure the concentrations of nicotine and cotinine in hair of smokers and non-smokers to evaluate their self-reported smoking and exposure to environmental tobacco smoke. In both cases, the method provided good selectivity, accuracy and precision. |
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Authors:
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Thaneeya Chetiyanukornkul; Akira Toriba; Ryoichi Kizu; Kazuko Kimura; Kazuichi Hayakawa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 18 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-03 Completed Date: 2005-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 655-61 Citation Subset: IM |
Copyright Information:
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2004 John Wiley & Sons, Ltd. |
Affiliation:
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Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Liquid
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methods* Cotinine / analysis* Hair / chemistry* Humans Nicotine / analysis* Reference Standards Smoke* Spectrometry, Mass, Electrospray Ionization / methods* Tobacco* Tobacco Smoke Pollution* |
| Chemical | |
Reg. No./Substance:
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0/Smoke; 0/Tobacco Smoke Pollution; 486-56-6/Cotinine; 54-11-5/Nicotine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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