| Hageman factor and its binding sites are present in senile plaques of Alzheimer's disease. | |
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MedLine Citation:
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PMID: 7987673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hageman factor (HF) or factor XII participates in several defense systems of the body. These include coagulation, fibrinolysis and complement activation. We investigated the expression of HF and its mRNA in control and Alzheimer's disease (AD) brain, using immunohistochemistry and polymerase-chain reaction (PCR) techniques. HF mRNA was detected in control and AD brain extracts, indicating that HF can be produced by endogenous brain cells. HF-like immunoreactivity was present in residual serum of capillaries in both control and AD brain, consistent with its known presence in the circulation. In addition, AD senile plaques were stained. The staining was dramatically enhanced when AD sections were incubated with solutions containing HF, indicating that plaques contain not only HF but also binding sites for HF. The enhanced staining was eliminated by pretreatment of solutions with the HF-binding agent kaolin. It was also eliminated by pretreatment of sections with protamine, an agent which strongly binds to negative surfaces. These data suggest that negatively charged surfaces in plaques might bind HF in vivo. Since HF can be activated by contact with negative surfaces, locally released HF could be playing a role in initiating a variety of inflammatory responses in AD brain. |
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Authors:
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O Yasuhara; D G Walker; P L McGeer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research Volume: 654 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-01-09 Completed Date: 1995-01-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 234-40 Citation Subset: IM |
Affiliation:
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Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Alzheimer Disease / metabolism*, pathology Base Sequence Binding Sites Brain / metabolism*, pathology* Capillaries / metabolism, pathology Cerebral Cortex / metabolism Cerebrovascular Circulation DNA Primers Factor XII / analysis, biosynthesis* Gene Expression Humans Immunohistochemistry Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger / analysis, biosynthesis Receptors, Cell Surface / analysis, biosynthesis* Reference Values Temporal Lobe / metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Messenger; 0/Receptors, Cell Surface; 9001-30-3/Factor XII |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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