Document Detail


Haemolysin-deficient variants of Streptococcus pyogenes and S. dysgalactiae subsp. equisimilis may be overlooked as aetiological agents of pharyngitis.
MedLine Citation:
PMID:  10966229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Variants of large colony beta-haemolytic Lancefield group A, C and G streptococci that are non-haemolytic or alpha-haemolytic on sheep blood agar have been detected in clinical specimens due to their enhanced haemolytic activity when grown on a new selective and differential blood agar medium containing colistin, nalidixic acid and pH 7.5-adjusted PIPES buffer (CNA-P). The large colony Lancefield group C and G isolates were identified as Streptococcus dysgalactiae subsp. equisimilis by API 20 Strep classification and 16S rDNA profiling. The haemolytic activity of these variants on various blood agar media, including CNA-P, was closely similar to that of known streptolysin S-defective mutants of S. pyogenes and was blocked by addition of cholesterol, a specific inhibitor of the streptolysin O family of haemolysins. As haemolysin variants could be detected in large numbers in cultures from patients with clinical symptoms of pharyngitis it is suggested that they may function as primary pathogens in such infections. The high frequency with which haemolysin variants were isolated from clinical specimens during a 3-month trial (3%, 13% and 10%, respectively, of group A, C and G streptococcal isolates) indicated that a substantial proportion of streptococcal infections may go undetected if only conventional sheep blood agar media are used in clinical laboratories for the detection of beta-haemolytic streptococci. As haemolysin variants have been implicated in the development of serious streptococcal sequelae, further investigation of the full extent of their contribution to streptococcal disease is indicated.
Authors:
K P Dierksen; J R Tagg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  49     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-09-08     Completed Date:  2000-09-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  811-6     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial*
Bacterial Outer Membrane Proteins*
Bacterial Proteins / genetics
Carrier Proteins / genetics
Culture Media
DNA, Ribosomal / genetics
Genetic Variation*
Hemolysin Proteins / genetics*
Hemolysis
Humans
Hydrogen-Ion Concentration
Pharyngitis / etiology*,  microbiology
RNA, Ribosomal, 16S / genetics
Streptococcus / classification,  genetics,  pathogenicity*
Streptococcus pyogenes / classification,  genetics,  pathogenicity
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Culture Media; 0/DNA, Ribosomal; 0/Hemolysin Proteins; 0/RNA, Ribosomal, 16S; 0/streptococcal M protein

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