| Haemolysin II is a Bacillus cereus virulence factor that induces apoptosis of macrophages. | |
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MedLine Citation:
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PMID: 20840334 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. The precise mechanisms and the bacterial factors allowing B. cereus to circumvent host immune responses remain to be elucidated. We have previously shown that B. cereus induces macrophage cell death by an unknown mechanism. Here we identified the toxic component from the B. cereus supernatant. We report that Haemolysin II (HlyII) provokes macrophage cell death by apoptosis through its pore-forming activity. The HlyII-induced apoptotic pathway is caspase 3 and 8 dependent, thus most likely mediated by the death receptor pathway. Using insects and mice as in vivo models, we show that deletion of hlyII strongly reduces virulence. In addition, we show that after infection of Bombyx mori larvae, the immune cells are apoptotic, demonstrating that HlyII induces apoptosis of phagocytic cells in vivo. Altogether, our results clearly unravel HlyII as a novel virulence protein that induces apoptosis in phagocytic cells in vitro and in vivo. |
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Authors:
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Seav-Ly Tran; Elisabeth Guillemet; Maud Ngo-Camus; Cyril Clybouw; Andrea Puhar; Arnaud Moris; Michel Gohar; Didier Lereclus; Nalini Ramarao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-9-14 |
Journal Detail:
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Title: Cellular microbiology Volume: - ISSN: 1462-5822 ISO Abbreviation: - Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-9-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2010 Blackwell Publishing Ltd. |
Affiliation:
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INRA, Unité MICALIS, UMR 1319, équipe GME, La Minière, 78285 Guyancourt, France. INSERM U542, Villejuif, France. Unité PMM, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France. INSERM UMRS-945, UPMC, Hôpital La Pitié-Salpêtrière, 75641 Paris, France. INRA, Unité MICALIS, UMR 1319, équipe RGBB, BP01, 78850 Thiverval-Grignon, France. |
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