Document Detail


Haemoglobin inhibition of fibrin polymerization and clotting.
MedLine Citation:
PMID:  8180334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clotting of human plasma by human alpha-thrombin was prolonged in the presence of haemoglobin as was human and bovine fibrinogen. Specifically, the clot time doubled for human plasma, human fibrinogen and bovine fibrinogen at 483, 233, and 116 microM haemoglobin, respectively. Fibrinopeptide A release was not inhibited at concentrations in approximately 16,000 molar excess compared with alpha-thrombin. Turbidometric analysis of fibrin polymerization showed a lengthening of the lag phase as well as the fibrin assembly process in the presence of haemoglobin. These findings suggested that neither fibrinogen recognition nor catalytic efficiency of thrombin was affected, implying that haemoglobin interferes with fibrin polymerization. Since human blood contains sufficient haemoglobin in erythrocytes to generate concentrations of up to 2.3 mM upon cell lysis, and haemoglobin concentrations of 0.16-0.48 mM caused 1.25 to two times longer clotting times in fresh human plasma, respectively, haemoglobin may act to modulate clot formation under conditions of haemolysis.
Authors:
D V Brezniak; D G Moon; J A Beaver; J W Fenton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis     Volume:  5     ISSN:  0957-5235     ISO Abbreviation:  Blood Coagul. Fibrinolysis     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-06-10     Completed Date:  1994-06-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9102551     Medline TA:  Blood Coagul Fibrinolysis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-43     Citation Subset:  IM    
Affiliation:
New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany 12201.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Coagulation / drug effects*
Cattle
Fibrin / metabolism*
Fibrinogen / pharmacology
Fibrinopeptide A / metabolism
Hemoglobins / pharmacology*
Humans
Kinetics
Nephelometry and Turbidimetry
Polymers
Thrombin / pharmacology
Grant Support
ID/Acronym/Agency:
HL 40615/HL/NHLBI NIH HHS; HL13160/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobins; 0/Polymers; 25422-31-5/Fibrinopeptide A; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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