| Haemoglobin O Padova and falsely low haemoglobin A1c in a patient with type I diabetes. | |
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MedLine Citation:
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PMID: 9215129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycated haemoglobin (HbA1c) measured by high performance liquid chromatography (HPLC) in a 20 year old female with insulin dependent diabetes mellitus was consistently within the normal range although her daily blood glucose values were > 11.1 mmol/l. HbA1c measured by immunoagglutination and fructosamine was elevated and correlated with the patient's blood glucose values. The HPLC chromatogram showed an additional peak at HbA0. Electrophoresis of haemoglobin on citrate agar gel revealed an abnormal haemoglobin anodal of HbS. Cellulose acetate electrophoresis and isoelectric focusing demonstrated an additional haemoglobin migrating close to HbA2. Amino acid analysis and DNA sequencing revealed an alpha 30 (B11) Glu-->Lys replacement, that is, haemoglobin O Padova. Investigations of two family members without diabetes revealed the same rare haemoglobin variant. This case showed that this silent haemoglobin mutation caused an additional peak and falsely low HbA1c values when measured by HPLC, the gold standard for this evaluation. |
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Authors:
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W J Schnedl; E C Reisinger; S Katzensteiner; R W Lipp; F Schreiber; P Hopmeier; G J Krejs |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: Journal of clinical pathology Volume: 50 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 1997 May |
Date Detail:
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Created Date: 1997-07-24 Completed Date: 1997-07-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 434-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Internal Medicine, Karl Franzens, University, Graz, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biological Markers / blood Chromatography, High Pressure Liquid Diabetes Mellitus, Type 1 / blood* False Negative Reactions Female Hemoglobin A, Glycosylated / analysis* Hemoglobins, Abnormal / analysis* Humans |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Hemoglobin A, Glycosylated; 0/Hemoglobins, Abnormal; 88358-63-8/hemoglobin O Padova |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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