| Haemin-binding proteins of Porphyromonas gingivalis W50 grown in a chemostat under haemin-limitation. | |
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MedLine Citation:
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PMID: 7504069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Porphyromonas gingivalis W50 was grown in a chemostat at pH 7.3 under haemin-limitation and haemin-excess at a constant mean doubling time of 6.9 h. Outer membranes (OM) were extracted from whole cells using EDTA and compared by SDS-PAGE. Haemin-limited cells expressed novel outer membrane proteins (OMPs) of mol. mass 115, 113 and 19 kDa when samples were solubilized at 100 degrees C. A 46 kDa OMP was observed in haemin-excess cells but not in those from haemin-limited conditions. Tetramethylbenzidine (TMBZ) staining of gels, after OM solubilization at 20 degrees C, was used to detect haemin-binding proteins (HBPs). HBPs were observed only in OM from haemin-limited cells. The major HBP (mol. mass 32.4 kDa) corresponded to a similar sized Kenacid-blue-stained protein which was not observed in haemin-excess-derived OM. Haemin-limited cells and OM displayed a ladder-like series of Kenacid-blue-stained proteins. Lighter TMBZ-stained proteins of mol. mass 51, 53, 56 and 60 kDa, with mobilities corresponding to those of silver-stained LPS components, were observed in haemin-limited OM. No soluble HBPs were detected extracellularly. The greater number of HBPs expressed by cells grown under haemin-limitation may reflect an additional cell surface receptor system for haemin acquisition under low environmental levels of this essential cofactor. |
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Authors:
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J W Smalley; A J Birss; A S McKee; P D Marsh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of general microbiology Volume: 139 ISSN: 0022-1287 ISO Abbreviation: J. Gen. Microbiol. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-12-29 Completed Date: 1993-12-29 Revised Date: 2005-01-21 |
Medline Journal Info:
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Nlm Unique ID: 0375371 Medline TA: J Gen Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2145-50 Citation Subset: IM |
Affiliation:
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Department of Clinical Dental Sciences, University of Liverpool, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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isolation & purification,
metabolism* Benzidines Biological Transport, Active Carrier Proteins / isolation & purification, metabolism* Cell Wall / metabolism Electrophoresis, Polyacrylamide Gel Hemeproteins / isolation & purification, metabolism* Hemin / metabolism* Porphyromonas gingivalis / growth & development, metabolism* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Benzidines; 0/Carrier Proteins; 0/Hemeproteins; 0/heme-binding protein; 16009-13-5/Hemin; 54827-17-7/3,3',5,5'-tetramethylbenzidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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