Document Detail


Haemangiomas are formed by cells expressing high levels of alphavbeta3 integrin and lacking acetylated LDL uptake.
MedLine Citation:
PMID:  15141386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Haemangiomas are benign tumours occurring in up to 12% of Caucasians, particularly in infancy and childhood. In the present study, two variant cell lines were isolated from murine endothelioma cells. One variant, named t.End.1V(high), represented 16.9% of the parental cell population and was selected by virtue of high expression levels of integrin alphavbeta3 and reduced capacity to endocytose acetylated low-density lipoproteins (Ac-LDLs). A second variant, named t.End.1V(low), represented 38.8% of the parental endothelioma cell line, expressed low levels of alphavbeta3 integrin, and was able to endocytose Ac-LDL. These phenotypic modifications were stable and correlated with specific morphological and functional properties of the two variant cell lines. While the t.End.1V(high) cells induced the formation of large haemangiomas when injected subcutaneously into mice, the t.End.1V(low) cells formed haemangiocytomas. When compared with t.End.1V(low) cells, the t.End.1V(high) cells showed increased migratory capacity, lacked an inflammatory response, and formed cord-like structures in fibrin gels. In contrast, the t.End.1V(low) cells organized into cysts with a lumen in fibrin gels. They rarely formed blood-filled haemangiomas in vivo and recruited host smooth muscle cells, a phenomenon typical for vessel wall maturation of resting cells. These data suggest that Ac-LDL uptake and the level of alphavbeta3 integrin expression are linked to the ability of endothelial cells to form large haemangiomas in vivo.
Authors:
Michel Aurrand-Lions; Caroline Johnson-Leger; Michael S Pepper; Beat A Imhof
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pathology     Volume:  203     ISSN:  0022-3417     ISO Abbreviation:  J. Pathol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-13     Completed Date:  2004-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204634     Medline TA:  J Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  700-9     Citation Subset:  IM    
Copyright Information:
Copyright 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Affiliation:
Department of Pathology, University Medical Centre, Geneva, Switzerland. Lions@medecine.unige.ch
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Cell Movement / physiology
Culture Media
Endothelial Cells / chemistry,  metabolism,  pathology
Endothelium, Vascular / chemistry,  metabolism,  pathology
Enzyme Inhibitors / metabolism
Fibrin / metabolism
Hemangioma / chemistry*,  metabolism,  pathology
Integrin alphaVbeta3 / analysis*
Lipoproteins, LDL / metabolism*
Mice
Muscle, Smooth, Vascular / chemistry*,  metabolism,  pathology
Chemical
Reg. No./Substance:
0/Culture Media; 0/Enzyme Inhibitors; 0/Integrin alphaVbeta3; 0/Lipoproteins, LDL; 0/acetyl-LDL; 9001-31-4/Fibrin

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