Document Detail


HY251, a novel cell cycle inhibitor isolated from Aralia continentalis, induces G1 phase arrest via p53-dependent pathway in HeLa cells.
MedLine Citation:
PMID:  19095450     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was aimed to elucidate the novel structure of HY251 isolated from the roots of Aralia continentalis and to evaluate its detailed inhibition mechanisms on cell cycle progression in HeLa cells. The structure of HY251 was elucidated based on the interpretation of the NMR spectra, as 3-propyl-2-vinyl-1,2,3,3a,3b,6,7,7a,8,8a-decahydrocyclopenta[a]indene-3,3a,7a,8a-tetraol. The flow cytometric analysis revealed an appreciable G(1) phase arrest in HeLa cells treated with 100 microM of HY251. This HY251-induced G(1) phase arrest is associated with decreased expression of cyclin D3 and up-regulation of p21(CIP1) and p27(KIP1), via p53 phosphorylation at Ser-15 by transcriptional up-regulation of ATM, which resulted in increased hypophosphorylated pRb in HeLa cells.
Authors:
Ha Lim Oh; Haeyoung Lim; Youl-Hee Cho; Hyun Chul Koh; Hojung Kim; Yoongho Lim; Chul-Hoon Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-27
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  19     ISSN:  1464-3405     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2010-07-06     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  959-61     Citation Subset:  IM    
Affiliation:
Department of Medical Genetics, College of Medicine, Hanyang University, 17 Haengdang-dong, Sungdong-gu, Seoul 133-791, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology
Aralia / metabolism*
Cell Cycle / drug effects*
Cell Cycle Proteins / metabolism
Chemistry, Pharmaceutical / methods
DNA-Binding Proteins / metabolism
Drug Screening Assays, Antitumor / methods
G1 Phase / drug effects*
Hela Cells
Humans
Indenes / chemistry,  pharmacology*
Magnetic Resonance Spectroscopy
Models, Chemical
Phosphorylation
Plant Extracts / metabolism,  pharmacology*
Protein-Serine-Threonine Kinases / metabolism
Tumor Suppressor Protein p53 / metabolism*
Tumor Suppressor Proteins / metabolism
Vinyl Compounds / chemistry,  pharmacology*
Chemical
Reg. No./Substance:
0/3-propyl-2-vinyl-1,2,3,3a,3b,6,7,7a,8,8a-decahydrocyclopenta(a)indene-3,3a,7a,8a-tetraol; 0/Antineoplastic Agents; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Indenes; 0/Plant Extracts; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 0/Vinyl Compounds; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein

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