| HV-1-specific cytotoxic T lymphocytes and breast milk HIV-1 transmission. | |
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MedLine Citation:
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PMID: 19434932 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Breast-feeding by infants exposed to human immunodeficiency virus type 1 (HIV-1) provides an opportunity to assess the role played by repeated HIV-1 exposure in eliciting HIV-1-specific immunity and in defining whether immune responses correlate with protection from infection. METHODS: Breast-feeding infants born to HIV-1-seropositive women were assessed for HLA-selected HIV-1 peptide-specific cytotoxic T lymphocyte interferon (IFN)-gamma responses by means of enzyme-linked immunospot (ELISpot) assays at 1, 3, 6, 9, and 12 months of age. Responses were deemed to be positive when they reached > or = 50 HIV-1-specific sfu/1 x 10(6) peripheral blood mononuclear cells (PBMCs) and were at least twice those of negative controls. RESULTS: A total of 807 ELISpot assays were performed for 217 infants who remained uninfected with HIV-1 at approximately 12 months of age; 101 infants (47%) had at least 1 positive ELISpot result (median, 78-170 sfu/1 x 10(6) PBMCs). The prevalence and magnitude of responses increased with age (P = .01 and P = .007, respectively); the median log(10) value for HIV-1-specific IFN-gamma responses increased by 1.0 sfu/1 x 10(6) PBMCs/month (P < .001) between 1 and 12 months of age. Of 141 HIV-1-uninfected infants with 1-month ELISpot results, 10 (7%) acquired HIV-1 infection (0/16 with positive vs. 10/125 [8%] with negative ELISpot results; P = .6). Higher values for log(10) HIV-1-specific spot-forming units at 1 month of age were associated with a decreased risk of HIV-1 infection, adjusted for maternal HIV-1 RNA level (adjusted hazard ratio, 0.09 [95% confidence interval, 0.01-0.72]). CONCLUSION: . Breast-feeding HIV-1-exposed uninfected infants frequently had HIV-1-specific IFN-gamma responses. Greater early HIV-1-specific IFN-gamma responses were associated with decreased HIV-1 acquisition. |
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Authors:
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Grace C John-Stewart; Dorothy Mbori-Ngacha; Barbara Lohman Payne; Carey Farquhar; Barbra A Richardson; Sandra Emery; Phelgona Otieno; Elizabeth Obimbo; Tao Dong; Jennifer Slyker; Ruth Nduati; Julie Overbaugh; Sarah Rowland-Jones |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 199 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-05-12 Completed Date: 2009-05-29 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 889-98 Citation Subset: AIM; IM |
Affiliation:
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Department of Paediatrics, University of Nairobi, Nairobi, Kenya. gjohn@u.washington.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acquired Immunodeficiency Syndrome
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microbiology,
transmission* Female HIV-1 / isolation & purification* Humans Infant Infant, Newborn Infectious Disease Transmission, Vertical* Interferon-gamma / immunology Milk, Human / virology* Pregnancy Pregnancy Complications, Infectious / virology* T-Lymphocytes, Cytotoxic / virology* |
| Grant Support | |
ID/Acronym/Agency:
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D43 TW000007/TW/FIC NIH HHS; K01 AI087369/AI/NIAID NIH HHS; K24 HD054314/HD/NICHD NIH HHS; K24 HD054314-04/HD/NICHD NIH HHS; P30 AI027757/AI/NIAID NIH HHS; R01 HD-23412/HD/NICHD NIH HHS; R01 HD023412/HD/NICHD NIH HHS; R01 HD023412-13/HD/NICHD NIH HHS; R01 HD023412-14/HD/NICHD NIH HHS; R01 HD023412-15/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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