Document Detail


Huh-7 or HepG2 cells: which is the better model for studying human apolipoprotein-B100 assembly and secretion?
MedLine Citation:
PMID:  20956548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apolipoprotein-B100 (apoB100) is the essential protein for the assembly and secretion of very low density lipoproteins (VLDL) from liver. The hepatoma HepG2 cell line has been the cell line of choice for the study of synthesis and secretion of human apoB-100. Despite the general use of HepG2 cells to study apoB100 metabolism, they secrete relatively dense, lipid-poor particles compared with VLDL secreted in vivo. Recently, Huh-7 cells were adopted as an alternative model to HepG2 cells, with the implicit assumption that Huh-7 cells were superior in some respects of lipoprotein metabolism, including VLDL secretion. In this study we addressed the hypothesis that the spectrum of apoB100 lipoprotein particles secreted by Huh-7 cells more closely resembles the native state in human liver. We find that Huh-7 cells resemble HepG2 cells in the effects of exogenous lipids, microsomal triglyceride transfer protein (MTP)-inhibition, and proteasome inhibitors of apoB100 secretion, recovery, and degradation. In contrast to HepG2 cells, however, MEK-ERK inhibition does not correct the defect in VLDL secretion. Huh-7 cells do not appear to offer any advantages over HepG2 cells as a general model of human apoB100-lipoprotein metabolism.
Authors:
Steven J R Meex; Ursula Andreo; Janet D Sparks; Edward A Fisher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-18
Journal Detail:
Title:  Journal of lipid research     Volume:  52     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-06-21     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  152-8     Citation Subset:  IM    
Affiliation:
Department of Medicine (Leon H. Charney Division of Cardiology), New York University School of Medicine, New York, NY 10016, USA.
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MeSH Terms
Descriptor/Qualifier:
Apolipoprotein B-100 / metabolism*
Cell Line
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors,  metabolism
Hep G2 Cells
Humans
Lipoproteins, VLDL / metabolism
Models, Biological
Grant Support
ID/Acronym/Agency:
HL58541/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoprotein B-100; 0/Lipoproteins, VLDL; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases
Comments/Corrections

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