Document Detail

HTLV-I Tax induces and associates with Crk-associated substrate lymphocyte type (Cas-L).
MedLine Citation:
PMID:  15592516     Owner:  NLM     Status:  MEDLINE    
Crk-associated substrate lymphocyte type (Cas-L) is a docking protein that is heavily tyrosine phosphorylated by the engagement of beta1 integrins in T cells. In the present study, we attempted to evaluate the role of Cas-L in the pathophysiology of adult T-cell leukemia (ATL). Examination of peripheral blood mononuclear cells from ATL patients as well as ATL-derived T cell lines showed an elevation of Cas-L in these cells. We showed that tyrosine phosphorylation as well as expression of Cas-L was markedly elevated through the induction of human T-lymphotropic virus type I (HTLV-I) Tax in JPX-9 cells, with these cells showing marked motile behavior on the ligands for integrins. We next performed yeast two-hybrid screening of cDNA library from an HTLV-I-transformed T cell line, which resulted in the identification of Tax as a putative binding partner for Cas-L. Co-precipitation experiments revealed that the serine-rich region of Cas-L might serve as the binding site with the highest affinity for Tax. Co-localization study showed that Tax and Cas-L partly merged in the cytoplasm. Finally, we showed that exogenous Cas-L inhibited Tax-mediated transactivation of nuclear factor kappaB (NF-kappaB), while Tax-independent activation of NF-kappaB remained intact, hence indicating that Cas-L might specifically regulate Tax-NF-kappaB pathway.
Satoshi Iwata; Akiko Souta-Kuribara; Akio Yamakawa; Takahiro Sasaki; Takatsune Shimizu; Osamu Hosono; Hiroshi Kawasaki; Hirotoshi Tanaka; Nam H Dang; Toshiki Watanabe; Naomichi Arima; Chikao Morimoto
Related Documents :
9600696 - In vitro and in vivo transformation in rat tracheal epithelial cells exposed to diesel ...
2833026 - Expression of the control elements of bk and sv40 viruses in human cells exhibiting dif...
6577446 - Molecular cloning and cell cycle-specific regulation of a functional human thymidine ki...
2177646 - Ebv utilizes a unique activation pathway for the transformation of human b cells.
1306296 - Dna repair pathways in mammalian cells analyzed by isolation of acnu-sensitive chinese ...
1924296 - The position of the microtubule-organizing center in directionally migrating fibroblast...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  24     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-11     Completed Date:  2005-03-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  1262-71     Citation Subset:  IM    
Division of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptor Proteins, Signal Transducing
Cadmium Chloride / pharmacology
Cell Line, Transformed
Cell Movement / genetics,  physiology
Cytosol / chemistry
Gene Products, tax / analysis,  metabolism*
Human T-lymphotropic virus 1 / metabolism
Leukemia, T-Cell / virology*
Leukocytes, Mononuclear / chemistry,  drug effects,  physiology
NF-kappa B / physiology
Phosphoproteins / analysis,  metabolism,  physiology*
Transcriptional Activation / physiology
Two-Hybrid System Techniques
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Gene Products, tax; 0/NEDD9 protein, human; 0/NF-kappa B; 0/Phosphoproteins; 10108-64-2/Cadmium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Aberrant methylation profile of human malignant mesotheliomas and its relationship to SV40 infection...
Next Document:  Low expression of Wnt-5a gene is associated with high-risk neuroblastoma.