Document Detail

HTLV-1a tax gene and long terminal repeat sequences from Argentinean strains reveal disagreement with tax restriction fragment length polymorphism subtyping.
MedLine Citation:
PMID:  17919108     Owner:  NLM     Status:  MEDLINE    
Sequence and cluster analysis have shown two HTLV-1a tax gene subgroups, tax A and tax B, which are related to long terminal repeat (LTR) molecular subtypes. On the basis of subgroup-specific nucleotide substitutions, restriction fragment length polymorphism (RFLP) analysis of the tax gene for subtyping HTLV-1a isolates was proposed. In this study we genetically characterized the tax gene from 63 HTLV-1-positive Argentinean individuals, including 14 patients with HTLV-1-associated myelopathy/tropical spastic paraparesis and 49 healthy HTLV-1 carriers. RFLP analysis showed that 48 samples yielded the tax A profile (76.19%) and that 15 samples contained the uncut tax B profile (23.81%). However, the LTR and tax sequence analysis revealed that in fact only 2 from the 15 samples belonged to the HTLV-1aB subgroup, presenting four tax B subgroup-specific nucleotide substitutions. The tax gene cluster analysis also confirmed that the majority of Argentinean strains belonged to the Transcontinental HTLV-1aA subgroup. These results indicate that the tax gene RFLP assay which has been proposed and used by some authors to screen HTLV-1a subgroups, is not a suitable tool to perform molecular epidemiological characterization of HTLV-1a populations.
Alena M Iñiguez; Koko Otsuki; Rene Gastaldello; Sandra Gallego; Ana Carolina P Vicente
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  23     ISSN:  0889-2229     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-10-08     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1127-30     Citation Subset:  IM; X    
Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ182355;  DQ182356;  DQ182358;  DQ182359;  DQ182360;  DQ182361;  DQ182362;  DQ182363;  DQ182364;  DQ182365;  DQ182366;  DQ182367;  DQ182368;  DQ182369;  DQ182370;  DQ182371;  DQ182372;  DQ182374;  DQ182375;  DQ182376;  DQ182377;  DQ182378;  DQ182379;  DQ182380;  DQ182381;  DQ182382;  DQ182383;  DQ182384;  DQ182385;  DQ182386;  DQ182387;  DQ182388;  DQ182389;  DQ182390;  DQ182391;  DQ182392;  DQ182393;  DQ182394;  DQ182395;  DQ182396;  DQ182397;  DQ182398;  DQ182399;  DQ182400;  DQ182401;  DQ182402;  DQ182403;  DQ182404;  DQ182405;  DQ182406;  DQ182407;  DQ182408;  DQ182409;  DQ182410;  DQ182411;  DQ182412;  DQ182413;  DQ182415;  DQ227129;  DQ227130;  DQ227131;  DQ227132;  DQ227133;  DQ227134;  DQ227135;  DQ227136;  DQ227137;  DQ227138;  DQ227139;  DQ227140;  DQ227141;  DQ227142;  DQ227143;  DQ227144;  DQ227145;  DQ227146;  DQ227147;  DQ227148;  DQ227149;  DQ227150;  DQ227151;  DQ227152;  DQ227153;  DQ227154;  DQ227155;  DQ227156;  DQ227157;  DQ227158;  DQ227159;  DQ227160;  DQ227161;  DQ227162;  DQ227163;  DQ227164;  DQ227165;  DQ227166;  DQ227167;  DQ227168;  DQ227169;  DQ227170;  DQ227171;  DQ227172;  DQ227173;  DQ227174;  DQ227175;  DQ227176;  DQ227177;  DQ227178;  DQ227179;  DQ227180;  DQ227181;  DQ227182;  DQ227183;  DQ227184;  DQ227185;  DQ227186;  DQ227187;  DQ227188;  DQ227189;  DQ227190;  DQ227191
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MeSH Terms
Gene Products, tax / chemistry,  genetics*
Genes, pX / genetics
HTLV-I Infections / virology
Human T-lymphotropic virus 1 / classification*,  genetics
Molecular Sequence Data
Paraparesis, Tropical Spastic / virology
Polymorphism, Restriction Fragment Length*
Sequence Analysis, DNA
Terminal Repeat Sequences / genetics*
Reg. No./Substance:
0/Gene Products, tax; 0/tax protein, Human T-lymphotrophic virus 1

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