| HTLV-1 viral load variability and long-term trends in asymptomatic carriers and in patients with HTLV-1-related diseases. | |
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MedLine Citation:
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PMID: 22894552 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: HTLV-1 proviral load (PVL) in peripheral blood mononuclear cells (PBMC) is high in patients with Adult T-cell Leukaemia/Lymphoma and HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis and in some asymptomatic carriers, but fluctuates. Objectives: To document ranges of HTLV PVL across a broader spectrum of diseases and tissues; to quantify the normal range of intra-patient PVL variability; to identify which PVL values and changes deserve further investigation. Methods: PVL was measured in 191 patients with HTLV-1-associated diseases and in 211 asymptomatic carriers, using real-time quantitative PCR. Results: The intra-assay variability increases as viral load decreases: 8% at high load, 17% at medium load, and 33% at low load. The inter-assay variability is not different from the intra-assay. Mean intra-patient CV is 65% (SD 21) in asymptomatic carriers, and 59% (SD 22) in HAM/TSP. PVL values varied widely between individuals, but were relatively constant within individuals. High PVL in cerebrospinal fluid (CSF) and lymph nodes (LN) was associated with disease but 57% of asymptomatic carriers had a PVL greater than 1% in PBMCs. Conclusion: Our results suggest that: 1) PVL changes falling outside a coefficient of variation of 100% require more detailed assessment; 2) asymptomatic carriers with PVL higher than 10% should undergo more frequent clinical and laboratory monitoring; 3) HTLV-1 PVL in blood and tissue is helpful in the diagnosis and monitoring of HTLV-1 infection. |
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Authors:
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Maria Antonietta Demontis; Silva Hilburn; Graham Taylor |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-15 |
Journal Detail:
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Title: AIDS research and human retroviruses Volume: - ISSN: 1931-8405 ISO Abbreviation: AIDS Res. Hum. Retroviruses Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709376 Medline TA: AIDS Res Hum Retroviruses Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Imperial College London, Infectious Diseases, London, United Kingdom; m.demontis@imperial.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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