Document Detail


HTLV-1 viral load variability and long-term trends in asymptomatic carriers and in patients with HTLV-1-related diseases.
MedLine Citation:
PMID:  22894552     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: HTLV-1 proviral load (PVL) in peripheral blood mononuclear cells (PBMC) is high in patients with Adult T-cell Leukaemia/Lymphoma and HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis and in some asymptomatic carriers, but fluctuates. Objectives: To document ranges of HTLV PVL across a broader spectrum of diseases and tissues; to quantify the normal range of intra-patient PVL variability; to identify which PVL values and changes deserve further investigation. Methods: PVL was measured in 191 patients with HTLV-1-associated diseases and in 211 asymptomatic carriers, using real-time quantitative PCR. Results: The intra-assay variability increases as viral load decreases: 8% at high load, 17% at medium load, and 33% at low load. The inter-assay variability is not different from the intra-assay. Mean intra-patient CV is 65% (SD 21) in asymptomatic carriers, and 59% (SD 22) in HAM/TSP. PVL values varied widely between individuals, but were relatively constant within individuals. High PVL in cerebrospinal fluid (CSF) and lymph nodes (LN) was associated with disease but 57% of asymptomatic carriers had a PVL greater than 1% in PBMCs. Conclusion: Our results suggest that: 1) PVL changes falling outside a coefficient of variation of 100% require more detailed assessment; 2) asymptomatic carriers with PVL higher than 10% should undergo more frequent clinical and laboratory monitoring; 3) HTLV-1 PVL in blood and tissue is helpful in the diagnosis and monitoring of HTLV-1 infection.
Authors:
Maria Antonietta Demontis; Silva Hilburn; Graham Taylor
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-15
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  -     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Imperial College London, Infectious Diseases, London, United Kingdom; m.demontis@imperial.ac.uk.
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