Document Detail

The HTLV-1 hbz antisense gene indirectly promotes tax expression via down-regulation of p30(II) mRNA.
MedLine Citation:
PMID:  21176937     Owner:  NLM     Status:  MEDLINE    
Human T-cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor (HBZ) is transcribed from the antisense genomic DNA strand and functions differently in its RNA and protein forms. To distinguish between the roles of hbz mRNA and HBZ protein, we generated mutants in a proviral clone that specifically disrupt the hbz gene product. A proviral clone with a splice acceptor mutation that disrupts expression of the predominant hbz mRNA resulted in lower levels of tax mRNA. Heterologous hbz expression restored Tax activity in cells expressing this mutant clone. In contrast, proviral mutants that disrupt HBZ protein did not affect levels of tax mRNA. Expression of hbz resulted in lower levels of p30(II) mRNA. Mutation of p30(II) overcame the effects of the splice acceptor mutation of hbz, and restored tax expression. Thus, there is a complex interplay of viral regulatory proteins controlling levels of HTLV-1 gene expression.
Gunjan Choudhary; Lee Ratner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-12-21
Journal Detail:
Title:  Virology     Volume:  410     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-28     Completed Date:  2011-03-09     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  307-15     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Basic-Leucine Zipper Transcription Factors / genetics,  metabolism*
Cell Line
Gene Expression Regulation, Viral
Gene Knockout Techniques
Gene Products, tax / biosynthesis*
Genetic Complementation Test
Human T-lymphotropic virus 1 / physiology*
Proviruses / genetics
RNA, Messenger / biosynthesis
RNA, Viral / biosynthesis
Retroviridae Proteins / biosynthesis*
Transcription, Genetic
Viral Proteins / genetics,  metabolism*
Grant Support
CA10073/CA/NCI NIH HHS; CA63417/CA/NCI NIH HHS; CA94056/CA/NCI NIH HHS; P01 CA100730-09/CA/NCI NIH HHS; P50 CA094056/CA/NCI NIH HHS; P50 CA094056-11/CA/NCI NIH HHS; R01 CA063417/CA/NCI NIH HHS; R01 CA063417-12/CA/NCI NIH HHS
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/Gene Products, tax; 0/HBZ protein, human T-cell leukemia virus type I; 0/RNA, Messenger; 0/RNA, Viral; 0/Retroviridae Proteins; 0/Viral Proteins; 0/tax protein, Human T-lymphotrophic virus 1; 0/tof protein, Human T-lymphotropic virus 1

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