Document Detail


HTII-280, a biomarker specific to the apical plasma membrane of human lung alveolar type II cells.
MedLine Citation:
PMID:  20566753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pulmonary alveolar epithelium is composed of two morphologically distinct cell types, type I (TI) and type II (TII) cells. Alveolar TII cells synthesize, secrete, and recycle surfactant components; contain ion transporters; and secrete immune effector molecules. In response to alveolar injury, TII cells have the capacity to act as progenitor cells, proliferating and transdifferentiating into TI cells. Although various proteins are associated with TII cells, a plasma membrane marker specific to human TII cells that would be useful for identification in tissue and for isolating this cell type has not been described previously. We devised a strategy to produce a monoclonal antibody (MAb) specific to the apical surface of human TII cells and developed an MAb that appears to be specific for human TII cells. The antibody recognizes a 280- to 300-kDa protein, HTII-280, which has the biochemical characteristics of an integral membrane protein. HTII-280 is detected by week 11 of gestation and is developmentally regulated. HTII-280 is useful for isolating human TII cells with purities and viabilities >95%. HTII-280 is likely to be a useful morphological and biochemical marker of human TII cells that may help to advance our understanding of various lung pathological conditions, including the origin and development of various lung tumors.
Authors:
Robert F Gonzalez; Lennell Allen; Linda Gonzales; Philip L Ballard; Leland G Dobbs
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-21
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  58     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2010-10-06     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  891-901     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / isolation & purification,  metabolism
Biological Markers / metabolism
Cell Membrane / metabolism
Cell Separation
Gene Expression Regulation, Developmental
Humans
Immunohistochemistry
Infant
Isoelectric Focusing
Membrane Proteins / chemistry,  immunology,  metabolism*
Mice
Mice, Inbred BALB C
Molecular Weight
Pulmonary Alveoli / embryology,  growth & development,  metabolism*
Respiratory Mucosa / metabolism
Solubility
Grant Support
ID/Acronym/Agency:
HL-086323/HL/NHLBI NIH HHS; HL-099820/HL/NHLBI NIH HHS; HL-24075/HL/NHLBI NIH HHS; HL-57426/HL/NHLBI NIH HHS; R01 HL086323/HL/NHLBI NIH HHS; R01 HL086323-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Membrane Proteins
Comments/Corrections

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