| HTF: A b-ZIP transcription factor that is closely related to the human XBP/TREB5 and is activated by hepatocellular carcinoma in rats. | |
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MedLine Citation:
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PMID: 8687468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We screened for rat hepatocellular carcinoma (HCC)-related genes by a novel cDNA subtraction method and obtained one gene. This gene was transcribed as 2.0- and 2.5-kb mRNAs, and its transcription was specifically enhanced in HCC. These cDNAs had the same open reading frame, but the 2.5 kb transcript had an extra 495 bases of 5'-UTR at the 5'-terminus. The deduced aa sequence revealed a basic-leucine zipper (b-ZIP) and proline/glutamine-rich structures, both of which are characteristic motifs for transcription factors. We designated the translation product of this gene HTF (Hepatocarcinogenesis-related Transcription Factor). Electrophoretic mobility shift assay demonstrated the DNA-binding ability of the recombinant HTF. It is most interesting that HTF had a considerable homology with human XBP/TREB5, which has been reported to be a binding factor for the X-box of the MHC class II gene and for the 21-bp enhancer of the HTLV-1 LTR. Genomic Southern analysis suggested that the 2.0- and 2.5-kb mRNAs are transcribed by a dual promoter of a single gene. Our results may suggest that HTF is a b-ZIP-type transcription factor involved in rat hepatocellular carcinoma. |
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Authors:
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T Kishimoto; K Kokura; Y Kumagai; T Wakamatsu; Y Makino; T Tamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 223 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-08-16 Completed Date: 1996-08-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 746-51 Citation Subset: IM |
Affiliation:
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Department of Biology, Faculty of Science, Chiba University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Basic-Leucine Zipper Transcription Factors Binding Sites Carcinoma, Hepatocellular / metabolism* Cloning, Molecular DNA, Complementary / chemistry, metabolism DNA-Binding Proteins / biosynthesis*, chemistry* Glutamine Humans Leucine Zippers Liver / metabolism* Liver Neoplasms / metabolism* Molecular Sequence Data Neoplasm Proteins* Nuclear Proteins / chemistry* Oligodeoxyribonucleotides Open Reading Frames Proline RNA, Messenger / biosynthesis Rats Recombinant Proteins / biosynthesis, chemistry Transcription Factors / biosynthesis*, chemistry* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Basic-Leucine Zipper Transcription Factors; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Transcription Factors; 0/regulatory factor X transcription factors; 147-85-3/Proline; 56-85-9/Glutamine |
| Comments/Corrections | |
Erratum In:
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Biochem Biophys Res Commun 2002 Sep 20;297(2):425 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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