| HSP70 production and inhibition of cell proliferation in Molt-4 T-cells after cell-to-cell transmission of HTLV-I: effect of PGA1. | |
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MedLine Citation:
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PMID: 7769835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infection with HTLV-I is associated with leukemic transformation of mature CD4+ T lymphocytes. PGA1, a powerful inhibitor of tumour cell proliferation, can prevent the clonal expansion of HTLV-I-infected cells following acute infection of cord blood-derived mononuclear cells. Since the antiproliferative effect of PGA1 on HTLV-I transformed, chronically infected MT-2 cell line was associated with induction of HSP70, we have investigated the effect of PGA1 on cell cycle progression and HSP70 production in a leukemic T-cell line (Molt-4) shortly after exposure to HTLV-I in a cell-to-cell transmission model. Rate of cell proliferation and HSP70 expression were studied within one duplication cycle of Molt-4 cells after exposure to HTLV-I. Growth of both control and virus-exposed cultures was inhibited by treatment with PGA1 (4 micrograms/ml) and cell cycling was arrested preferentially at the G1/S interphase. Synthesis of HSP70 was induced within 3 h by PGA1 in control and virus-exposed Molt-4 cells and became undetectable from overnight onward, though the protein accumulated in the cells. The arrest of growth was observed from overnight up to 48 h so that treated cells almost missed one cycle. Interestingly, HSP70 transcript and protein persisted at remarkably high levels in Molt-4 cells exposed to HTLV-I in the absence of PGA1, showing that HSP70 expression can be directly activated during primary infection with this human retrovirus. Moreover, in these cocultures, treatment with PGA1 or heat shock was not able to increase further the elevated level of HSP70 found in untreated cocultures, suggesting that during the early period of the virus-transmission phase, HTLV-I could interfere with HSP70 induction by other inducers. |
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Authors:
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C D'Onofrio; A Puglianiello; C Amici; I Faraoni; G Lanzilli; E Bonmassar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leukemia research Volume: 19 ISSN: 0145-2126 ISO Abbreviation: Leuk. Res. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-07-05 Completed Date: 1995-07-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7706787 Medline TA: Leuk Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 345-56 Citation Subset: IM; X |
Affiliation:
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Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Compartmentation Cell Division / drug effects Cell Line HTLV-I Infections / metabolism*, transmission Heat-Shock Proteins / biosynthesis*, metabolism Hot Temperature Humans Prostaglandins A / pharmacology* T-Lymphocytes / metabolism, microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 0/Prostaglandins A; 14152-28-4/prostaglandin A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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