Document Detail


HSP70 is associated with endothelial activation in placental vascular diseases.
MedLine Citation:
PMID:  18372927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endothelial cell injury and activation in the placenta are features of placental vascular disease (PVD). While advances in PVD have been made, the pathogenesis of this disease is still unknown. The objective of this study was to pursue potential risk factors and signal transcription pathways involved in PVD pathogenesis. Gene expression in subjects with PVD and with normal pregnancies was compared using a two-channel microarray technique. Higher expression of HSPA6 and HSPA1A was exhibited in PVD subjects. HSPA6 and HSPA1A both encode HSP70, and, therefore, we localized HSP70 expression in placental tissue. Using quantitative polymerase chain reaction (PCR) and Western blot, we observed a significant upregulation of HSP70 in both mRNA and protein levels in placental tissue and microvascular endothelial cells of PVD subjects when compared with normal pregnancies (P< 0.05). HSP70 mRNA and protein expression also correlated negatively with infant birth weight (P< 0.05). HSP70 was expressed mainly in endothelial cells and smooth muscle cells in the placental microvessels. We therefore conclude that HSP70 may mediate endothelial activation and play a role in pathogenesis of PVD.
Authors:
Yanxia Liu; Nannan Li; Li You; Xin Liu; Hongyan Li; Xin Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular medicine (Cambridge, Mass.)     Volume:  14     ISSN:  1528-3658     ISO Abbreviation:  Mol. Med.     Publication Date:    2008 Sep-Oct
Date Detail:
Created Date:  2008-09-24     Completed Date:  2008-11-10     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9501023     Medline TA:  Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  561-6     Citation Subset:  IM    
Affiliation:
Department of Hematology, Provincial Hospital affiliated to Shandong University, Jinan, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Birth Weight
Blotting, Western
Endothelial Cells
Endothelium, Vascular / metabolism,  physiopathology*
Female
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Humans
Infant, Newborn
Microcirculation
Oligonucleotide Array Sequence Analysis
Placenta / blood supply
Placenta Diseases / metabolism,  physiopathology*
Polymerase Chain Reaction
Pregnancy
RNA, Messenger / genetics,  metabolism
Up-Regulation*
Vascular Diseases / metabolism,  physiopathology*
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/RNA, Messenger
Comments/Corrections

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