| HSP110, caspase-3 and -9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all-trans retinoic acid or irradiation during early mouse eye development. | |
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MedLine Citation:
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PMID: 17451530 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potential role in apoptosis. The aim of this research was to study the expression of HSP110, caspase-3 and -9 in physiological, retinoic- or irradiation-induced apoptosis during early eye development. Seven pregnant C57Bl/6J mice received 80 mg kg(-1) of all-trans retinoic acid mixed with sesame oil. Seven pregnant NMRI mice received 2 Gy irradiation at the same gestational day. Control mice of both strains (seven mice of each) were not submitted to any treatment. Embryos were harvested at 3, 6, 12 and 24 h after exposition, fixed, dehydrated and embedded. Coronal sections (5 microm) were made. Slide staining occurred alternatively using anti-caspase-3, anti-caspase-9 and anti-HSP110 immunohistochemistry. HSP110 and caspase-3 expression presented similar topographic and chronological patterns, whereas expression of HSP110 was more precocious in retinoic acid-treated embryos. After retinoic exposure, caspase-3- and HSP110-positive cells were increased in the region of the optic vesicle. By contrast, after irradiation, caspase-3- and HSP110-positive cells were noticeably increased in the optic vesicle, peri-optical mesoderm but less in lens placode. HSP110 was expressed before caspase-3. By contrast, caspase-9 was expressed by a very small number of cells in the optic vesicle either under physiological or under teratogenic conditions. Thus, it seems that activation of caspase-9 is dispensable in early eye developmental apoptosis. |
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Authors:
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Julien Gashegu; Reza Ladha; Nathalie Vanmuylder; Catherine Philippson; Françoise Bremer; Marcel Rooze; Stéphane Louryan |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of anatomy Volume: 210 ISSN: 0021-8782 ISO Abbreviation: J. Anat. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-24 Completed Date: 2007-09-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0137162 Medline TA: J Anat Country: England |
Other Details:
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Languages: eng Pagination: 532-41 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Embryology, Université Libre de Bruxelles, Faculty of Medicine, Brussels, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abnormalities, Drug-Induced
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metabolism Abnormalities, Radiation-Induced / metabolism Animals Apoptosis / drug effects, radiation effects Caspase 3 / analysis* Caspase 9 / analysis* Embryonic Development / drug effects, physiology*, radiation effects Eye / drug effects, embryology*, radiation effects Female Gestational Age HSP110 Heat-Shock Proteins / analysis* Immunohistochemistry Mice Mice, Inbred C57BL Mice, Mutant Strains Models, Animal Pregnancy Teratogens / pharmacology Tretinoin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/HSP110 Heat-Shock Proteins; 0/Teratogens; 302-79-4/Tretinoin; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
| Comments/Corrections | |
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