Document Detail


HSP 27 as possible prognostic factor in patients with oral squamous cell carcinoma.
MedLine Citation:
PMID:  14702179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HSP27 belongs to the Heat shock protein (HSP) family, which plays essential functions in cells under physiological conditions and prevents stress-induced cellular damage. The aim of this study was to investigate the biological role of HSP27 in oral tumorigenesis. MATERIALS AND METHODS: Seventy-nine cases of oral squamous cell carcinoma and 10 cases of normal mucosa were analysed for HSP27 expression by immunohistochemistry. Moreover, the western blot analysis was performed on two cases of normal mucosa and five cases of OSCC. RESULTS: Normal oral mucosa showed a suprabasal expression of HSP27. Twenty-four cases of SCC (30.7%) showed a diffuse staining for HSP27, and 48 cases (60.3%) showed instead a decrease in staining, which was diffuse, homogeneous, or with alternation of positive and negative areas in a single tumor ("mosaic" pattern). Only 7 cases of OSCC (7.5%) were completely negative for HSP27. Frequency of lymph node metastases was higher in HSP27-negative tumours (3/7, 42.8%) than in HSP-reduced (16/48, 33.3%) or positive ones (5/26, 19.2%). Regard staging, stages I and II had a higher score than stages III and IV (stage I > stage II > stage III > stage IV). There was also a statistically significant correlation between HSP27 expression and grade: HSP27 expression was reduced in poorly differentiated tumours (P < 0.05). When analysed for prognostic significance, patients with negative/reduced HSP27 expression had poorer survival rates than the group with positive HSP27 expression (P < 0.05). The statistical analysis of these findings showed no significant correlation between HSP27 expression, sex, and tumour size. CONCLUSION: Cases with reduced expression were more aggressive and poorly differentiated. These data suggest that HSP27 expression may be useful in order to identify cases of oral squamous cell carcinoma with more aggressive and invasive phenotype providing novel diagnostic and prognostic information on individual patient survival with oral cancers.
Authors:
L Lo Muzio; R Leonardi; M A Mariggiò; M D Mignogna; C Rubini; A Vinella; G Pannone; L Giannetti; R Serpico; N F Testa; G De Rosa; S Staibano
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  19     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-31     Completed Date:  2004-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  119-28     Citation Subset:  IM    
Affiliation:
Institute of Dental Sciences, University of Ancona, Ancona, Italy. lomuziol@tin.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Blotting, Western
Carcinoma, Squamous Cell / diagnosis*,  mortality,  pathology*
Female
Heat-Shock Proteins / diagnostic use*
Humans
Immunohistochemistry
Italy
Lymphatic Metastasis
Male
Middle Aged
Mouth Neoplasms / diagnosis*,  mortality,  pathology*
Neoplasm Staging
Prognosis
Survival Rate
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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