Document Detail

HPRT activity in embryos of a South American opossum Monodelphis domestica.
MedLine Citation:
PMID:  7878225     Owner:  NLM     Status:  MEDLINE    
Marsupial females show preferential paternal X-inactivation. However, the time at which X-inactivation occurs in early development has not yet been determined. A double microassay which measures the activities of X-linked hypoxanthine phosphoribosyl transferase (HPRT) and the autosomally-coded adenine phosphoribosyl transferase (APRT) from the same sample was performed on a collection of embryos from a South American opossum Monodelphis domestica. The embryos ranged in age from the 2-cell stage to the bilaminar blastocyst stage. The results indicate that their embryonic HPRT and APRT are not expressed until just before the unilaminar blastocyst stage in M. domestica. This is at a later stage of development than that in the mouse where embryonic HPRT and APRT expression first occurs at the 4-8-cell stage. It is concluded that HPRT is an uniformative enzyme for assessing X chromosome activity in cleaving embryos of M. domestica. The widespread distribution of HPRT:APRT ratios after the unilaminar blastocyst stage also makes it difficult to draw conclusions about the state of X chromosome activity in early marsupial development.
P G Johnston; D Dean; J L VandeBerg; E S Robinson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  6     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  1994  
Date Detail:
Created Date:  1995-04-06     Completed Date:  1995-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  529-32     Citation Subset:  IM    
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
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MeSH Terms
Adenine Phosphoribosyltransferase / metabolism
Dosage Compensation, Genetic
Hypoxanthine Phosphoribosyltransferase / metabolism*
Opossums / embryology*
Reg. No./Substance:
EC Phosphoribosyltransferase; EC Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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