Document Detail


HPLC imprinted-stationary phase prepared by precipitation polymerisation for the determination of thiabendazole in fruit.
MedLine Citation:
PMID:  16284658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A molecularly imprinted polymer (MIP) tailored for the HPLC determination of the fungicide thiabendazole (TBZ) has been synthesised in one single preparative step by precipitation polymerisation in an acetonitrile/toluene co-solvent, using TBZ as template molecule, methacrylic acid as functional monomer and divinylbenzene-80 as crosslinker. The imprinted polymer particulates obtained were characterised by scanning electron microscopy and nitrogen sorption porosimetry. These analyses showed clearly that spherical polymer particulates (polymer microspheres) with narrow size distributions (average particle diameter approximately 3.5 microm) and well-developed pore structures had been produced. The imprinted microspheres were packed into a stainless steel HPLC column (50 x 4.6 mm id) and evaluated as an imprinted stationary phase. The imprinting effect was demonstrated clearly, i.e., the column was observed to bind TBZ selectively, and the effect of different chromatographic parameters (e.g., temperature, flow-rate and elution solvents) on TBZ retention/elution studied. Under optimised conditions, the TBZ-imprinted column was used for the HPLC-fluorescence (HPLC-F) determination of TBZ directly from orange (both whole fruit and juice), lemon, grape and strawberry extracts at low concentration levels in less than 15 min, without any need for a clean-up step in the analytical protocol.
Authors:
E Turiel; J L Tadeo; P A G Cormack; A Martin-Esteban
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-14
Journal Detail:
Title:  The Analyst     Volume:  130     ISSN:  0003-2654     ISO Abbreviation:  Analyst     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-14     Completed Date:  2006-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1601-7     Citation Subset:  IM    
Affiliation:
Departamento de Medio Ambiente, INIA, Carretera de A Coruña km 7.5, E-28040 Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Antinematodal Agents / analysis*
Chromatography, High Pressure Liquid / methods
Food Analysis / methods*
Fruit / chemistry*
Microscopy, Electron, Scanning
Pesticide Residues / analysis*
Thiabendazole / analysis*
Chemical
Reg. No./Substance:
0/Antinematodal Agents; 0/Pesticide Residues; 148-79-8/Thiabendazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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