| HPLC detection of soluble carbohydrates involved in mannitol and trehalose metabolism in the edible mushroom Agaricus bisporus. | |
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MedLine Citation:
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PMID: 10691629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A convenient and sensitive method was developed to separate and detect various types of carbohydrates (polyols, mono- and disaccharides, and phosphorylated sugars) simultaneously using high-performance liquid chromatography (HPLC). The method consists of a chromatographic separation on a CarboPac PA1 anion-exchange analytical column followed by pulsed amperometric detection. In a single run (43 min) 13 carbohydrates were readily resolved. Calibration plots were linear over the ranges of 5-25 microM to 1. 0-1.5 mM. The reliable and fast analysis technique, avoiding derivatization steps and long run times, was used to determine the levels of carbohydrates involved in mannitol and trehalose metabolism in the edible mushroom Agaricus bisporus. Moreover, the method was used to study the trehalose phosphorylase reaction. |
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Authors:
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W J Wannet; J H Hermans; C van Der Drift; H J Op Den Camp |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 48 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-04-24 Completed Date: 2000-04-24 Revised Date: 2002-11-08 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 287-91 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agaricales
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metabolism* Carbohydrates / chemistry* Chromatography, High Pressure Liquid / methods Glucosyltransferases / metabolism Mannitol / metabolism* Plants, Edible / metabolism Solubility Trehalose / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 69-65-8/Mannitol; 99-20-7/Trehalose; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.64/alpha,alpha-trehalose phosphorylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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