Document Detail

HPLC based activity profiling for 5-lipoxygenase inhibitory activity in Isatis tinctoria leaf extracts.
MedLine Citation:
PMID:  15893435     Owner:  NLM     Status:  MEDLINE    
In the pursuit of the anti-inflammatory constituents in lipophilic woad extracts, the 5-lipoxygenase (5-LOX) inhibitory activity was investigated by HPLC-based activity profiling. In a low-resolution profiling, two time windows with peaks of activity were found. The first coincided with tryptanthrin, a known dual inhibitor of cyclooxygenase-2 (COX-2) and 5-LOX, whereas the major inhibitory fraction was towards the end of the HPLC run. The active fractions were profiled in a peak-resolved manner, and the compounds analyzed by LC-MS, GC and TLC. The activity in the lipophilic fractions of the Isatis extract could be linked to an unsaturated fatty acid, alpha-linolenic acid.
C Oberthür; R Jäggi; M Hamburger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Fitoterapia     Volume:  76     ISSN:  0367-326X     ISO Abbreviation:  Fitoterapia     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-31     Completed Date:  2005-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  16930290R     Medline TA:  Fitoterapia     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  324-32     Citation Subset:  IM    
Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, D-07741 Jena, Germany.
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MeSH Terms
Arachidonate 5-Lipoxygenase / antagonists & inhibitors*
Chromatography, High Pressure Liquid
HL-60 Cells
Isatis / chemistry*
Molecular Structure
Plant Extracts / chemistry,  pharmacology*
Plant Leaves / chemistry*
Quinazolines / chemistry,  pharmacology*
alpha-Linolenic Acid / chemistry
Reg. No./Substance:
0/Plant Extracts; 0/Quinazolines; 13220-57-0/tryptanthrine; 463-40-1/alpha-Linolenic Acid; EC 5-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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