Document Detail

HPLC analysis of underivatized fatty acids in margarines.
MedLine Citation:
PMID:  7142356     Owner:  NLM     Status:  MEDLINE    
The composition of the underivatized fatty acids in margarines was determined by high performance liquid chromatography (HPLC). A 50-min analysis gave not only the distribution of the cis- and trans-isomers of the 18 carbon-length, but also a quantitative measure of seven of the underivatized fatty acids studied. High concentrations of the essential fatty acid, linoleic acid, were found in the four margarine samples. Results of the quantitation, reproducability of the HPLC analysis, and stability of the standards are discussed. It was found that the developed HPLC analysis was able to adequately separate the geometric isomers, linoleic acid (18:2 delta 9c, 12c) from linolelaidic acid (18:2 delta 9t, 12t). However, a mixture of the other two isomers, 18:2 delta 9c, 12t and 18:2 delta 9c, 12c coeluted together between the peaks due to the 18:2 delta 9c, 12c and 18:2 delta 9t, 12t isomers. This study did not attempt to separate the various positional isomers of underivatized fatty acids.
A G Bailie; T D Wilson; R K O'Brien; J M Beebe; J D Stuart; E McCosh-Lilie; D W Hill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  20     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-01-19     Completed Date:  1983-01-19     Revised Date:  2001-03-23    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  466-70     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid
Fatty Acids / analysis*
Margarine / analysis*
Reg. No./Substance:
0/Fatty Acids; 8029-82-1/Margarine

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