Document Detail


HPLC analysis of reduced and oxidized coenzyme Q(10) in human plasma.
MedLine Citation:
PMID:  11159774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The percentage of reduced coenzyme Q(10) (CoQ(10)H(2)) in total coenzyme Q(10) (TQ(10)) is decreased in plasma of patients with prematurity, hyperlipidemia, and liver disease. CoQ(10)H(2) is, however, easily oxidized and difficult to measure, and therefore reliable quantification of plasma CoQ(10)H(2) is of clinical importance. METHODS: Venous blood was collected into evacuated tubes containing heparin, which were immediately placed on ice and promptly centrifuged at 4 degrees C. The plasma was harvested and stored in screw-top polypropylene tubes at -80 degrees C until analysis. After extraction with 1-propanol and centrifugation, the supernatant was injected directly into an HPLC system with coulometric detection. RESULTS: The in-line reduction procedure permitted transformation of CoQ(10) into CoQ(10)H(2) and avoided artifactual oxidation of CoQ(10)H(2). The electrochemical reduction yielded 99% CoQ(10)H(2). Only 100 microL of plasma was required to simultaneously measure CoQ(10)H(2) and CoQ(10) over an analytical range of 10 microg/L to 4 mg/L. Intra- and interassay CVs for CoQ(10) in human plasma were 1.2-4.9% across this range. Analytical recoveries were 95.8-101.0%. The percentage of CoQ(10)H(2) in TQ(10) was approximately 96% in apparently healthy individuals. The method allowed analysis of up to 40 samples within an 8-h period. CONCLUSIONS: This optimized method for CoQ(10)H(2) analysis provides rapid and precise results with the potential for high throughput. This method is specific and sufficiently sensitive for use in both clinical and research laboratories.
Authors:
P H Tang; M V Miles; A DeGrauw; A Hershey; A Pesce
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  47     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  256-65     Citation Subset:  IM    
Affiliation:
Division of Pediatric Neurology, The Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229-3039, USA. Tangp0@chmcc.org
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MeSH Terms
Descriptor/Qualifier:
Calibration
Chromatography, High Pressure Liquid
Coenzymes
Electrochemistry
Humans
Oxidation-Reduction
Reference Values
Sensitivity and Specificity
Ubiquinone / analogs & derivatives,  blood*,  chemistry
Chemical
Reg. No./Substance:
0/Coenzymes; 1339-63-5/Ubiquinone; 303-98-0/coenzyme Q10

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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