Document Detail


HPLC Methods for Determination of D: -Aspartate and N-methyl-D: -Aspartate.
MedLine Citation:
PMID:  21956568     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
D: -Amino acids are stereoisomers or optical isomers of naturally occurring L: -amino acids and thus possess the same chemical structure, but may differ in their biological/physiological properties. Until a half century ago, D: -amino acids had been considered to be unnatural substances found only in microorganisms. However, improvements in analytical instruments and methods have revealed that D: -amino acids are present in invertebrates and vertebrates, including humans, and that they possess important physiological functions. D: -Aspartate (D: -Asp) and its methylated form N-methyl-D: -aspartate (NMDA) possess neuroendocrine properties in many species. Several methods have been developed for determination of D: - and L: -enantiomers of amino acids by high performance liquid chromatography (HPLC). We report here improved HPLC methods for the specific determination of D: -Asp and NMDA in biological tissues.
Authors:
George H Fisher; Mara Tsesarskaia
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  253-64     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Barry University, Miami Shores, FL, USA, gfisher@mail.barry.edu.
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