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HPLC Determination of the Major Active Flavonoids and GC-MS Analysis of Volatile Components of Dysphania graveolens (Amaranthaceae).
MedLine Citation:
PMID:  23037638     Owner:  NLM     Status:  Publisher    
INTRODUCTION: Dysphania graveolens is used mainly in Mexican traditional medicine against gastrointestinal ailments. Previous investigations revealed that its flavonoids are important active principles; however, there is not a reliable and accurate analytical method for determining these compounds in the crude drug or preparations of the plant. In addition, its volatile chemical composition remains unknown. OBJECTIVE: The main goals were to develop a validated HPLC method for quantifying the active flavonoids (pinostrobin (1), pinocembrin (2) and chrysin (3)) of D. graveolens and to establish its volatile composition. METHODOLOGY: Separation was carried out on a Licrospher100 RP18 column with a linear gradient acetonitrile 0.1% formic acid and aqueous 0.1% formic acid. Accuracy was determined by spiking the crude drug with the standards, the recoveries were between 99% and 101%. A systematic description of the volatile components of D. graveolens was assessed via GC-MS using headspace solid-phase microextraction (HS-SPME) and hydrodistillation extraction methods. RESULTS: The developed HPLC method represented a powerful technique for the quality control of D. graveolens allowing the quantification of the three active flavonoids. For each compound a linear response was evaluated within the range of 0.5-2.0 mg/mL for pinostrobin (1), 0.25-1.25 mg/mL for pinocembrin (2) and 0.05-0.5 mg/mL for chrysin (3). According to SPME the major components in D. graveolens were p-cymene (84.85%) and eucalyptol (11.26%). On the other hand, the essential oil had eucalyptol (42.89%) and p-cymene (16.51%) and did not contain ascaridol. Thus the most relevant volatile components in the species were monoterpenoids. Copyright © 2012 John Wiley & Sons, Ltd.
Harry Alvarez-Ospina; Isabel Rivero Cruz; Georgina Duarte; Robert Bye; Rachel Mata
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Phytochemical analysis : PCA     Volume:  -     ISSN:  1099-1565     ISO Abbreviation:  Phytochem Anal     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200492     Medline TA:  Phytochem Anal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Facultad de Química, Universidad Nacional Autónoma de México, 04510, México, DF, México.
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