| HNK-1 expression pattern in normal and bis-diamine induced malformed developing rat heart: three dimensional reconstruction analysis using computer graphics. | |
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MedLine Citation:
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PMID: 1384394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The spatiotemporal distribution of the immunoreactivity of monoclonal antibody HNK-1 was investigated immunohistochemically in normal and bis-diamine-induced malformed rat embryonic hearts using three-dimensional reconstruction with computer graphics. First recognized in the primitive heart 11.5 days after conception, HNK-1 immunoreactivity was distributed in the atrio-ventricular and bulbo-ventricular junctional areas with incomplete ring-like appearance in the early embryonic stages. In the late embryonic stages the immunoreactive sites were rearranged and localized in the sites topographically corresponding to almost the entire pathway of the conduction system, including the three major internodal tracts connecting the right sinoatrial node and atrioventricular node. Immunoreactivity gradually decreased after the completion of the conduction system, and only a faint reactivity in the atrio-ventricular node region remained in the new-born heart. These results indicate that HNK-1 is expressed temporarily in the pathways corresponding to the conduction system during the development of the heart. In bis-(dichloroacethyl)-octamethylen-diamine (bis-diamine)-induced malformed hearts, localization of HNK-1 immunoreactivity was not remarkably altered in the early embryonic heart. In the late embryo, immunoreactive sites in the sino-atrial node region and atrio-ventricular node region deviated dorsocaudally with the poorly developed internodal tracts, and abnormal distribution was observed in the bilateral atria. We consider that these abnormalities may occur in conjunction with abnormal morphological development such as insufficient absorption of the sinus venosus. |
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Authors:
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H Ito; K Iwasaki; T Ikeda; H Sakai; I Shimokawa; T Matsuo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anatomy and embryology Volume: 186 ISSN: 0340-2061 ISO Abbreviation: Anat. Embryol. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-11-17 Completed Date: 1992-11-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7505194 Medline TA: Anat Embryol (Berl) Country: GERMANY |
Other Details:
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Languages: eng Pagination: 327-34 Citation Subset: IM |
Affiliation:
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First Department of Pathology, Nagasaki University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Antigens, CD57 Antigens, Differentiation / analysis* Diamines Female Heart / embryology*, growth & development* Heart Defects, Congenital / chemically induced, metabolism* Image Processing, Computer-Assisted* Immunohistochemistry Myocardium / chemistry* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD57; 0/Antigens, Differentiation; 0/Diamines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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