Document Detail

HMGA2 expression in uterine leiomyomata and myometrium: quantitative analysis and tissue culture studies.
MedLine Citation:
PMID:  12874787     Owner:  NLM     Status:  MEDLINE    
The high mobility group gene, HMGA2, is frequently expressed in uterine leiomyomata (UL) with chromosomal rearrangements of 12q15. In contrast, HMGA2 expression has not been detected in karyotypically normal UL or in myometrium, but has been detected in these tissues after culture. To characterize further the expression pattern of HMGA2, we assessed HMGA2 expression by RT-PCR followed by Southern blot hybridization, and by real-time PCR in three tissue panels: (1) primary myometrial cultures, (2) uncultured tissue from 15 karyotypically normal samples consisting of eleven 46,XX UL and four matched myometrial specimens, and (3) uncultured tissue from ten UL with 12q15 rearrangements and three matched myometrial specimens. HMGA2 expression was detected in all samples from the three panels. The level of HMGA2 expression in karyotypically normal UL was similar to the level of expression in myometrium; however, it was significantly less than the level measured in UL with 12q15 rearrangements. This expression analysis by use of detection methods of different sensitivities underscores the importance of studies of HMGA2 expression in uncultured tissues and of careful interpretation of results from experiments on cultured cells. Moreover, detection of HMGA2 expression in myometrium and in UL without 12q15 rearrangements, tissues previously thought not to express HMGA2, suggests that HMGA2 expression is required in normal adult myometrial physiology.
Karen L Gross; David M Neskey; Nitasha Manchanda; Stanislawa Weremowicz; Michael S Kleinman; Romana A Nowak; Azra H Ligon; Piere Rogalla; Klaus Drechsler; Jörn Bullerdiek; Cynthia C Morton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  38     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-07-22     Completed Date:  2003-10-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  68-79     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Pathology, University of Vermont, Burlington, Vermont, USA.
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MeSH Terms
Chromosomes, Human, Pair 12 / genetics
Culture Techniques
Gene Expression Regulation, Neoplastic / drug effects,  genetics*
Gene Rearrangement / genetics
HMGA2 Protein / analysis,  biosynthesis*,  genetics*
Leiomyoma / genetics*
Middle Aged
Myometrium / chemistry,  metabolism*,  pathology
Tumor Cells, Cultured
Uterine Neoplasms / genetics*
Grant Support
Reg. No./Substance:
0/HMGA2 Protein

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