| HLH-14 is a C. elegans achaete-scute protein that promotes neurogenesis through asymmetric cell division. | |
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MedLine Citation:
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PMID: 14627726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Achaete-Scute basic helix-loop-helix (bHLH) proteins promote neurogenesis during metazoan development. In this study, we characterize a C. elegans Achaete-Scute homolog, HLH-14. We find that a number of neuroblasts express HLH-14 in the C. elegans embryo, including the PVQ/HSN/PHB neuroblast, a cell that generates the PVQ interneuron, the HSN motoneuron and the PHB sensory neuron. hlh-14 mutants lack all three of these neurons. The fact that HLH-14 promotes all three classes of neuron indicates that C. elegans proneural bHLH factors may act less specifically than their fly and mammalian homologs. Furthermore, neural loss in hlh-14 mutants results from a defect in an asymmetric cell division: the PVQ/HSN/PHB neuroblast inappropriately assumes characteristics of its sister cell, the hyp7/T blast cell. We argue that bHLH proteins, which control various aspects of metazoan development, can control cell fate choices in C. elegans by regulating asymmetric cell divisions. Finally, a reduction in the function of hlh-2, which encodes the C. elegans E/Daughterless bHLH homolog, results in similar neuron loss as hlh-14 mutants and enhances the effects of partially reducing hlh-14 function. We propose that HLH-14 and HLH-2 act together to specify neuroblast lineages and promote neuronal fate. |
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Authors:
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C Andrew Frank; Paul D Baum; Gian Garriga |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. Date: 2003-11-19 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 130 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-08 Completed Date: 2004-04-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 6507-18 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3204, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Basic Helix-Loop-Helix Transcription Factors Body Patterning / genetics Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics, metabolism* Cell Division / physiology Conserved Sequence DNA Primers Embryo, Nonmammalian / physiology Gene Deletion Helix-Loop-Helix Motifs Molecular Sequence Data Motor Neurons / cytology, physiology Multigene Family Nervous System / cytology, embryology* Neurons / cytology, physiology Neurons, Afferent / cytology, physiology Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Transcription Factors / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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NS42213/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/Caenorhabditis elegans Proteins; 0/DNA Primers; 0/HLH-14 protein, C elegans; 0/HLH-2 protein, C elegans; 0/Transcription Factors |
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