Document Detail


HLA-targeted cell sorting of microchimeric cells opens the way to phenotypical and functional characterization.
MedLine Citation:
PMID:  22509428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microchimerism refers to the presence of less than 1% of non-host cells in a person. Our group developed a reliable method for separating viable microchimeric cells from the host environment. Optimal separation of microchimeric cells at proportions as low as 0.01% could be established with two monoclonal antibodies directed against different HLA antigens, one targeting the microchimeric cells and the other the host cells. Purity of separated cell populations was validated by HLA-allele-specific and Y-chromosome directed real-time qPCR assays. The methodology was used successfully to separate microchimeric maternal cells from child umbilical cord mononuclear cells after pregnancy. Cell sorting with HLA monoclonal antibodies targeting allelic differences enables reliable microchimeric cell detection and separation in blood specimens. With this approach, maximal enrichment of potentially viable microchimeric cells from a background cell population is reached, which opens the way to phenotypical and functional characterization of microchimeric cells.
Authors:
Michael Eikmans; Frans H J Claas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chimerism     Volume:  2     ISSN:  1938-1964     ISO Abbreviation:  Chimerism     Publication Date:    2011 Oct-Dec
Date Detail:
Created Date:  2012-04-17     Completed Date:  2013-01-24     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101540510     Medline TA:  Chimerism     Country:  United States    
Other Details:
Languages:  eng     Pagination:  114-6     Citation Subset:  IM    
Affiliation:
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands. m.eikmans@lumc.nl
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MeSH Terms
Descriptor/Qualifier:
Alleles
Antibodies, Monoclonal / immunology
Cell Separation / methods*
Chimerism*
Chromosomes, Human, Y
Female
HLA Antigens / genetics,  immunology*,  metabolism
Humans
Leukocytes, Mononuclear / cytology
Pregnancy
Umbilical Cord / cytology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/HLA Antigens
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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