Document Detail

HLA class-I-restricted and colon-specific cytotoxic T cells from lamina propria lymphocytes of patients with ulcerative colitis.
MedLine Citation:
PMID:  11811783     Owner:  NLM     Status:  MEDLINE    
We established cytotoxic T-lymphocyte (CTL) lines against colonic epithelial cell line from LPLs of patients with ulcerative colitis by continuous stimulation with human lymphocyte antigen A (HLA-A) matched colonic epithelial cell lines and clones from LPLs using polyclonal stimulation with phytohemagglutinin. The established CTL lines and clones showed cytotoxicity against HLA-A-matched colonic epithelial cell line but not against HLA-mismatched colonic epithelial cell lines, and HLA-A-matched lung and esophagus cell lines. The CTL response was HLA class I-restricted and mediated by CD8-positive T-lymphocytes. Moreover, the CTL line showed cytotoxicity against autologous B-LCLs pulsed with peptides extracted from HLA-A-matched colonic epithelial cell line but not against other organ-derived peptides pulsed and unpulsed autologous B-LCLs. CTL lines and clones established from LPLs of patients with ulcerative colitis showed colon-specific and HLA class I-restricted killing of human colonic epithelial cell line, suggesting that these CTLs may play a role in colonic epithelial cell damage in some patients with ulcerative colitis.
T Sunagawa; Y Yonamine; F Kinjo; M Watanabe; T Hibi; A Saito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical immunology     Volume:  21     ISSN:  0271-9142     ISO Abbreviation:  J. Clin. Immunol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2002-01-28     Completed Date:  2002-07-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102137     Medline TA:  J Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
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MeSH Terms
Aged, 80 and over
Cell Line
Colitis, Ulcerative / immunology*
Colon / immunology*
HLA-A Antigens / genetics,  immunology*
Intestinal Mucosa / immunology*
Middle Aged
T-Lymphocytes, Cytotoxic / immunology*
Reg. No./Substance:
0/HLA-A Antigens

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