Document Detail


HLA class II alleles and haplotypes in Lithuanian children with type 1 diabetes and healthy children (HLA and type 1 diabetes).
MedLine Citation:
PMID:  20966625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Type 1 diabetes mellitus is a slowly progressive autoimmune disease. The genetic background of type 1 diabetes mellitus is polygenic with the major disease locus located in the human leukocytes antigen (HLA) region. High risk and protective alleles, haplotypes, and genotypes have been determined in Lithuanian children with type 1 diabetes mellitus and healthy children.
MATERIAL AND METHODS: In this case-control study, 124 children with diabetes (55 males and 69 females; mean age, 9.2±3.9 years) were tested for HLA class II and compared with 78 healthy controls (43 males and 35 females; mean age, 10.8±3.4 years; range, 0-15 years). HLA DRB1, DQA1, and DQB1 alleles were genotyped using a polymerase chain reaction.
RESULTS: T1D risk-associated haplotypes (DR4)-DQA1*0301-DQB1*0302, (DR3)-DQA1*0501-DQB1*0201, and (DR1)-DQA1*0101-04-DQB1*0501 were more prevalent among children with diabetes than controls (50.0%, 41.1%, and 37.9% vs. 10.3%, 5.1%, and 24.4%, P<0.001). The haplotypes (DR4)-DQA1*0301-DQB1*0302 and (DR3)-DQA1*0501-DQB1*0201 increased T1D risk by 8.75 and 12.93 times, respectively (P<0.001). Protective haplotypes (DR2)-DQA1*0102-B1*0602, (DR11/12/13)-DQA1*05-DQB1*0301, and (DR13)-DQA1*0103-DQB1*0603 were significantly more prevalent among controls than children with diabetes (25.6%, 33.3%, 19.2% vs. 0%, 3.2%, 0%; P<0.001). These frequencies are quite similar to those from neighbor countries with varying incidence of type 1 diabetes mellitus.
CONCLUSIONS: HLA class II haplotypes associated with type 1 diabetes mellitus positively or negatively were the same in Lithuanian children as in other European Caucasian populations. Differences in incidence and clinical manifestations of type 1 diabetes might be due to different environmental factors and/or lifestyle.
Authors:
Erika Skrodenienė; Dalia Marčiulionytė; Zilvinas Padaiga; Edita Jašinskienė; Vaiva Sadauskaitė-Kuehne; Carani B Sanjeevi; Johnny Ludvigsson
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicina (Kaunas, Lithuania)     Volume:  46     ISSN:  1648-9144     ISO Abbreviation:  Medicina (Kaunas)     Publication Date:  2010  
Date Detail:
Created Date:  2010-10-22     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9425208     Medline TA:  Medicina (Kaunas)     Country:  Lithuania    
Other Details:
Languages:  eng     Pagination:  505-10     Citation Subset:  IM    
Affiliation:
Department of Preventive Medicine, Institute of Endocrinology, Kaunas University of Medicine, Eivenių 2, 50028 Kaunas, Lithuania. Erika.S@takas.lt
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Alleles
Case-Control Studies
Child
Child, Preschool
Data Interpretation, Statistical
Diabetes Mellitus, Type 1 / epidemiology*,  genetics*
Female
Genetic Predisposition to Disease
HLA Antigens* / immunology
Haplotypes
Humans
Incidence
Infant
Infant, Newborn
Life Style
Lithuania / epidemiology
Male
Polymerase Chain Reaction
Risk Factors
Statistics, Nonparametric
Chemical
Reg. No./Substance:
0/HLA Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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