Document Detail

HLA-DRneg patients without acute promyelocytic leukemia show distinct immunophenotypic, genetic, molecular, and cytomorphologic characteristics compared to acute promyelocytic leukemia.
MedLine Citation:
PMID:  19291801     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Loss of HLA-DR and CD34 is a well-known characteristic of malignant promyelocytes in acute promyelocytic leukemia (APL). However, this immunophenotype is not specific for APL. The purpose of this study was to investigate whether further biological characterization of the HLA-DR(neg) acute myeloid leukemia patients would allow more clearly define criteria to separate APL from non-APL patients. METHODS: Immunophenotyping, cytogenetics, molecular analyses, and cytomorphology were prospectively performed within routine leukemia diagnostics of 800 patients included in different prospective acute myeloid leukemia multicenter trials. RESULTS: Beside 60 APL, an additional 62 HLA-DR(neg) non-APL patients were identified. The main differential characteristics of HLA-DR(neg) non-APL included high CXCR-4 expression in most patients and almost all leukemia cells, a significantly higher proportion of patients presenting with NPM1 mutations as well as the significant association with cup-like nuclear morphology. The biological distinctness of both leukemia subtypes was further emphasized by the complete absence of aberrant CD2 expression and increased leukocyte and platelet counts in HLA-DR(neg) non-APL patients. Even in the CD34(pos) subgroup of HLA-DR(neg) non-APL all those features contributed in at least the same way to the separation from APL. CONCLUSIONS: The results of the present study show that an immunophenotypic, molecular, and cytomorphologic separation of both HLA-DR(neg) leukemia subgroups is possible indicating that both groups are biologically distinct.
Uta Oelschlaegel; Brigitte Mohr; Markus Schaich; Ulrike Schäkel; Frank Kroschinsky; Thomas Illmer; Gerhard Ehninger; Christian Thiede
Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study    
Journal Detail:
Title:  Cytometry. Part B, Clinical cytometry     Volume:  76     ISSN:  1552-4957     ISO Abbreviation:  Cytometry B Clin Cytom     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-10-13     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101235690     Medline TA:  Cytometry B Clin Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-7     Citation Subset:  IM    
Copyright Information:
(c) 2009 Clinical Cytometry Society.
University Hospital, University of Technics, Medical Clinic and Policlinic I, Dresden, Germany.
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MeSH Terms
Aged, 80 and over
Antigens, CD34 / metabolism
Blood Platelets / pathology
Cell Nucleus / metabolism,  pathology
Chromosome Banding
DNA, Neoplasm / analysis
Flow Cytometry / methods*
HLA-DR Antigens / metabolism*
Immunophenotyping / methods
Leukemia, Promyelocytic, Acute / genetics,  metabolism*,  pathology
Leukocytes / pathology
Middle Aged
Molecular Diagnostic Techniques
Nuclear Proteins / genetics,  metabolism
Prospective Studies
Receptors, CXCR4 / metabolism
Young Adult
fms-Like Tyrosine Kinase 3 / genetics,  metabolism
Reg. No./Substance:
0/Antigens, CD34; 0/CXCR4 protein, human; 0/DNA, Neoplasm; 0/HLA-DR Antigens; 0/Nuclear Proteins; 0/Receptors, CXCR4; 117896-08-9/nucleophosmin; EC protein, human; EC Tyrosine Kinase 3

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