Document Detail


HLA-DRB and -DBQ typing by PCR amplification using sequence-specific primers (PCR-SSP): assessment after 1 year of routine use by three laboratories.
MedLine Citation:
PMID:  9098425     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using sequence-specific amplifications, a practical and fast technique for DRB and DQB typing has been developed. The primers are chosen in order to amplify groups of alleles corresponding to the same serological specificity. In a second step, precise allelic determination is obtained by studying the restriction fragment length polymorphism of the PCR products. The experience of three laboratories using this technique in the context of organ or bone marrow transplantation is reported.
Authors:
B Mercier; R al Daccak; A Samaan; F David; A Carta; P Cracco; O Raguenes; F Dufosse; C Ferec; D Charron
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics     Volume:  21     ISSN:  0960-7420     ISO Abbreviation:  Eur. J. Immunogenet.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1997-05-14     Completed Date:  1997-05-14     Revised Date:  2010-10-15    
Medline Journal Info:
Nlm Unique ID:  9106962     Medline TA:  Eur J Immunogenet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  105-23     Citation Subset:  IM    
Affiliation:
Centre de Biogénétique, Brest, France.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Cell Line
DNA Primers*
HLA-DQ Antigens / classification,  genetics*
HLA-DR Antigens / classification,  genetics*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; 0/HLA-DQ Antigens; 0/HLA-DQB1 antigen; 0/HLA-DR Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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